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The Pathogen Biology And Control Of Eggplant Verticillium Wilt

Posted on:2004-06-14Degree:MasterType:Thesis
Country:ChinaCandidate:H ZhangFull Text:PDF
GTID:2133360095461651Subject:Vegetable science
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The paper researched on the biological character of the pathogen of eggplant verticillium wilt, disease resistance identification of eggplant varieties(lines), and control .The results showed:30 isolates of verticillium were collected or isolated from eggplant, pepper andcotton in Jiangsu Province. The original colony color of these isolates were black. However, the color and form of the colonies varied after be subcultured several times. For instance, some of the isolates losed the capbility of producing microsclerotium and their aerial mycelium changed dense or thin. These isolates were classified as three types by the variation capability and the stability of the variation: I Hard to vary, the isolates could keep the original character after be shifted 5times,4 isolates belonged to this type; II Easy to variance but the variant could return the original character,20 isolates belonged to this type;IIIEasy to variance and the variant were stable and couldn't return the original character, 6 isolates belonged to this type. Five isolates which included the three above types and isolated from different hosts were choosed for the test of culture and pathogenicity.The results showed that there was no various or significant difference among these isolates for the requirement of temperature and pH, themorphological variance hadn't significant relationship with pathogenicity. For example, the isolates with different color didn't showed significant different pathogenicity. The isolates from cotton showed different pathogenicity with the isolates from pepper and eggplant The pathogenicity of five isolates was different on eggplant ,pepper and cotton. Soluble proteins of verticillium dahliae were tractionated by polyacrylamide gel plate electrophoresis. The results showed the protein patterns were obvious difference among isolates obtained from different hosts and with different pathogenicity .But the protein patterns of the original were basic resemblance with it's variantThirteen eggplant varieties(lines) were tested for disease resistance. The resultshowed that none of them was immune,but the resistances varied among them :three varieties were medium resistant (15 < DI 30) , six varieties tolerant (30
Keywords/Search Tags:eggplant, verticillium dahliae, biological characteristic, control, electrophoresis
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