Study Of Sympthine And Betaine & Ricinine With Different Level On The Toxicity And Growth Of Mouse

This paper studied extraction, prepartion and determination methods of three kinds of common alkaloids(sympthine, betaine, ricinine)and determinated the effect of three alkaloids with different level on the toxicity and growth of mouse. Detemination's explortion of three kinds of common alkaloids. The experiment took sympthine ,betaine and recinine as example. The results showed gross alkaloid in sympthine root and leaf were 0.05g/100g and 0.030g/100g respectively by the way of mass methods . At the same time , we obtained results about colour and sediment reactions of alkaloid agent and sympthine. We also acquired recinine in husking castor bean, detoxic cake dregs and toxic cake dregs being 0.077%,0.224%,and 0.33% respectively by the way of organic solvent extraction and spectrophotemetry and got colour reactions of alkaloid agent and recinine . Meanwhile, we found betaine hydrochloric acid salt gotten by spectrophotemetry had liner relationship to do with absorptivity in concentration scope of 0.2~1.0mg/ml.Study about the effect of three alkaloids on the toxicity and growth of mouse. Mouse(waned) was choosed as object( body weigh range from 15 to 21g) and parted into four groups (n=18,per group). The experiment groups were fed different alkaloids with different level of ration(HD,MD,LD) .Sympthine were fed into the experiment groups at the doses of 0.25g/kg, 3.75g/kg and 5g/kg,betaine were 2.5g/kg,3.75g/kg and 5g/kg and recinine 0.05g/kg, 0.1g/kg and 0.3g/kg. The results showed within four week there was significant difference of gross body weigh gains between the controlled class and HD group(p<0.01) and feed intake and feed efficiency of mouse showed decreasing tendency in betaine group and sympthine group. At the same time, with the increase of adding dose of these two alkaloids, GPT and GOT of all experimental groups showed increasing tendency and had significant differencebetween all experimental groups and the controlled class(p<0.05),especially between HD group and the controlled class. There were tumid phenomena in pathologico-anatomic liver margin and epithelial cell of renal tubles in these two alkaloids groups. However, there was no pathological change in betaine groups.The above results suggested : (1) As to extraction and determination methods of the three alkaloids, mass method, spectrophotometry and spectrophotometry weresimple and fesible respectively. (2) As to toxicity and growth effect of three alkaloids with different level, sympthine at the lower dose level could promote the growth of mouse. However, when its proportion in fodder was more than 0.375g/kg,there was toxicity sign in mouse; ricinine at the middle and low dose level had a negative effect on the body weigh gains and feed intake of mouse. When its proportion in fodder was more than 0.3g/kg ,there was damage effect on the liver and renal of mouse. However ,betaine was not found toxicity on mouse.