Study On The Physiological And Biochemical Characters Of Suspension Culture Cell Of Camptotheca Acuminata Decsne

Camptothecin is a kind of anticancer medicine having good prospect of exploitation, CPT and analogue are get from CPA. at the moment, output of CPT was effected by the growth and environment of CPA. so production of CPT by cell culture may be a promising choice .In the paper ,based on callus of Camptotheca acuminata Decsne., the influences of light, hormones and several factors on the growth, physiological, biochemical characters of suspension culture of Camptotheca acuminata Decsne. Were systematically studied, cell growth speed was obviously improved, it would give references for future studies.1.Effects of hormonesDifferent kinds, different concentration and combinations of hormones had different effects on cell growth. 0.5mg/l NAA was optimal for the cell growth ; Among the combination of NAA and KT ,0.5mg/lNAA+0.1mg/lKT was optimal for the cell growth.2.Study of physiological and biochemical charactersWhen CPA was culture in the light and in the dark ,both of their cell growth curves were like "S", but their growth circles were about 30 and 27 days respectively ,It could be divided into lag phase, exponential growth phase and stationary phase .The cell grew more rapidly in the white light than in the dark ;The PH values of culture mediums lowered and then showed up gradually ;Two peaks of soluble protein contents and peroxides and superoxide dismutase activities of culture cell were showed up ,but they were on the different days ;the soluble protein content was higher in the white light.3.Effects of different physical and chemical factorsIn the suspension culture ,B5medium and White medium were optimal for cell growth ;The inoculums quantity of 3-4g @ FW/1001 was better for the cellgrowth ;The growth rate increased with the increasing concentrations of Sucrose and Glucose ;The medium with certain concentration of Agarose could increase the growth rate ; Effect of cultural cell growth was different with different proportion of NO3-and NH4+.Finally, the conditions in cell suspension culture of some medicine plants were analyzed ;research and development -, problems and resolutions were discussed; prospects of future researches were also given.