The Studies On The Cell Immobilized Of Ginkgo Biloba L. And The Production Of Ginkgolides

The studies on cell immobilization culture of Ginkgo biloba L, as well as the production of their secondary metabolite -Ginkgolides were reported in this paper. A cell line, which fit the request of the immobilization culture very well, was obtained by induction and optimized selection. The cell line grown quickly and well dispersed. The optimized result of cell immobilization cultures was acquired, including the support matrices pretreatment, the class and size of support matrices, the level of dissolved 62 and inoculum. The optimal ratio of hormones was got by uniform design according to its high immobilization level, high metabolism and retaining vigorous cells with long period. A medium which fit well all the conditions was obtained, and the efficiency of producing Ginkgolides by cell culture improved obviously.