| 22 crossed weaning piglets were used in this study,in order to sample and analyze for their diarrhea faecal ,intestines tissue and intestinal inclusion and surrounding blood . By isolating and calculating bectrium from collecting sample,it was conducted to research changes of intestinal normal microflora. After puritifying and identificating ,vitro restraining experiment by several kinds bacteria was done through the cross-over orthogonal and anti-orthogonal experiment. Surrounding blood was conducted to carry out the erythrocyte E-rosette assay and α-ANAE experiment ,so as to justify the level of T-cell immunity function . By pathogenic technology the intestinal membrane shape with weaning diarrhoea piglets were observed by optic microscope. The data was dealt with by SPSS(10.0 version)software. Results were as follow:1. Correlation between faecal condition and pH value Special correlation existed between faecal condition and pH value. At the diarrhea onset or little diarrhea ,pH value was acid ；faecal color was lighter than normal piglet. And pH value of the heavy diarrhea faecal, was over 7 with green. Faecal color was connected not only with this factor ,but also with feed qualification,therefore final color was not decided only by pH value high and low.2. Intestinal microflora changes:Most pathogenic bacteria in this research achieved growth more than usually on the medium culture , EMb,S.S,SP and so on ,but other minority lessened . The number of normal microflora, such as Intestinal Coccus, Bifidobacterium, Yeast, Intestinal bacterium, Lactobacillus et. declined, but there was no significant difference (p>0.05). The number of bacteroides declining was more significant difference than normal piglets (p<0.05). The results indicated that when piglets were diarrheaing proportion disorder of normal microflora had happened. So for changes ,different piglet had different result, but total trend with ratio between pathologenic and normal microflora significantly increased, even in individual piglet ratio got to 25 times. The ratios of E/L, E/B, S/L, S/B also did. And then in differentintestine ratios differed significantly (p<0.01). The minimul number had difference in twice isolated process.3. The external restraining experiment : Between bacteria respectively, there was different degree restraining role, including high sensitive , middle sensitive , low sensitive, no reaction. Different bacterium density displayed difference restraining activies.4. T-cell immunity function: E-rosette assay of surrounding blood from piglets with diarrhea differed significantly from normal piglets (p<0.01). Et positive decreased from 51% to 23%,Ea positive from 26% to 17.5%.α-ANAE positive of same piglets were also significant difference (p<0.01) from the health one ,decreasing from normally 40.5% to 32.7%. These justified that T-cell immunity function of piglet with diarrhea obviously decreased.5. Intestinal membrane shape change of piglet with weaning diarrhea The research also demonstrated that Iieum villus height from piglet with weaning diarrhea significantly lessen short at 18d, 28d, 50d, comparising with control individually and declining to 15%,18%,13%. The depth of intestinal gland at 18d,28d obviously deepened , change-increasing frequence individually 9 %,41%. But at 50d exception happened ,there was no depth change . So for the ratio of villus height /depth of intestinal gland, all results decreased with control at 18d, 28d, 50d. At 28d there was most obviously changed. Cecum gland also deepened 66%,44% than control at 28d,50d and more influence on effect than Iieum's. |
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