Generation, Selection And Identification Ways Of The Beet Translocation Lines Of An Apomictic Monosomic Addition Lines M14 Of Beta Corolliflora Zoss In Sugar Beet

Monosomic addition lines M14 of Beta corolliflora Zoss in sugar beet is a specific apomictic material of our country. It will facilitate the speed from genetic characters to gene cloning and function identifying that selection and characterization of the beet translocation lines with the fragment of chromosome from Beta corolliflora Zoss, which is very important to clone apomictic gene.The research accelerated the generation of translocation line with y -ray. Firstly, seeds of monosomic addition lines M14 were induced by y -ray, and then were planted in greenhouse.Secondly, The paper investigated the survival probability of seeds induced with different radiant intensity and had a cytological analysis by microscopical examination on surviving ones.Finally, this experiment made those plants have a dot hybridization by using the specific sequence 1054 of the Beta corolliflora Zoss as probe, of which chromosomes were aberrant. In that investigation, 11 translocation lines were found, and translocation line occurring frequency reached 4.54%, which obviously was more than that of spontaneous translocation and had a great effect on radiant inducement. The research results indicated that the intensity of -ray affect the survival probability of beet and the frequency of translocation line occurring deeply. The more strong radiant intensity was, the lower the survival probability of beet was, and the higher the frequency of chromosome differentiating and translocation line occurring was. According to that two factors, radiation with 60Kr and 80Kr had a better effect The experiment also had a research with dot hybridization to the 264 translocation plants of the 16 series, the research results indicated that the offsprings'general transfer-rate reached 50.4%, of which the transfer-rate of plant 11 and plant14 was up to 100% and 83.3%, belonging to high frequency transmission. Finally, a set of successful experiment project about fluorescence in situ hybridization was constructed through kinds of contrastive experiments which excluded spreading of plant chromosomes, denaturalization, hybridization,washing and detection of signals and so on.