Studies On Genetic Transformation Of Bt.CPTI Insect-Resistant Gene To Rice

Rice is the main foodstuff in the world and regarded as staple food by almost half of population. According to Stat. : The planting area of rice in our country occupy 28.9% of it in the world .The overall output occupy primacy in all foodstuff. But the production of rice is affected by lepidopteral insect. The reduction of output exceed 10% because of insect pest. The double insect-resistant gene can stave the mithridatism of insect and extend used time of transformed rice in this experiment. So the transformed rice can exert action plenty.In this experiment the double insect-resistant gene was transformed into Dongnong 419,Dongnong 420 and Dongsheng 6 those are the popularized breeds with the methods of co-cultivation with A.tumefaciens, microprojectile bombardment and electroporation. The effects of different kinds of co-cultivation on Perc.of resistant calli , the effect of different antioxidants on restoring growth state and browning inhibition of transformed rice calli, The effects of Enzyme and Filter pressure on separation of singer cell and the effect of different medium on the growth of rice suspended cell were studied. The medium that was used in singer cell-electroporation was established. The obtained transformed rice was proved that objective gene has been transformed in it by the GUS and PCR examine.