Effect Of Supplemental Copper Of Different Type On Nutrition And Immunity Of Mice

Microelement amino acid chelate is a newly additive, which was studied extensively, and the nutritional function of microelement amino acid was confirmed by many studies. But the study of copper amino acid chelate was seldom. In this study, mice were used as animal model, Cu-Lys and Cu-Met were studied as representative of copper amino acid chelate. Three experiments were conducted to study the effect of Copper Source and Supplemental Level in Diet on the Copper nutrition and immune function of mice.Trial 1, Effect of Supplemental Copper of different type on Nutrition and Specific Immunity of Mice- IAt the base of copper exhaustion for 20 days, 84 male KM mice were fed with the following feeds supplemented with different form and level copper: Cu-Lys4.5mg/kg (counted with copper, the following was same), Cu-Lys 45mg/kg, Cu-Met 4.5mg/kg, Cu-Met 45mg/kg, CuSO4 4.5mg/kg,CuSO4 45mg/kg, adding a copper deficiency diet ,there were 7 treatments totally, three repeats in each treatments. Those mice were fed in plastic cages, each repeat was fed respectively. Following the 7d,14d,21d,28d feeding period, in each treatment, one mouse was killed to count the ratio of peripheral ANAE+T lymphocyte, Serum IgG content, Serum Cu,Zn-SOD activity, Serum CP activity, Immune organ index , Copper concentration in Liver and Spleen.Trial 2, Effect of Supplemental Copper of different type on Nutrition and Specific Immunity of Mice- IIThe grouping of Trialt animal was the same as Trial 1, at the first day, second day, third day, one mouse was injected with PHA brine fluid for 10mg/kg avoirdupois after weighing in the same time in each repeat, following the 7d,14d,21d,8d feeding period, after weighing, blood was made, Wrigh-Giemsa coloration, counting the number of lymphocyte female cell and overage lymphocyte, index of immune organ, copper concentration in Liver and Spleen.Trial 3, Effect of Supplemental Copper of different type on No-Specific Immunity of MiceThe grouping of experiment animal was the same as Trial 1, at 7th,14th,21th,8th , one mouse was injected diluent Chinese ink for 10mg/kg avoirdupois with 4 times after weighing , blood was made from eyepit in time of second and 20th minute after injecting, killing the mouse immediately, weighing the Liver and Spleen, calculate the Correct phagocytic index.The previous 3 Trials were conducted to study the effect of 3 source of copper, 2 supplemental level and copper deficiency on copper nutrition status, immune function. Theresults of the experiment showed:(1) Mice's Liver Cu, Spleen Cu and Serum Cu were not affected significantly by dietary Cu Source, Mice's spleen Cu increased by dietary supplemental Copper, Serum Cu increased significantly, Liver Cu increased significantly in 45mg/kg Cu treatment.(2) Mice's activity of Cu,Zn-SOD and CP were not affected significantly by dietary Cu Source, Mice's activity of Cu,Zn-SOD and CP increased by dietary supplemental Copper.(3) Cu amino acid chelate promoted spleen growth in experiment 21-28d, thymus' degeneration were decreased and growth of spleen were promoted by supplemental Cu in diet in Cu-deficient mice.(4) Cu-Met promoted T lymphopoiesis more efficiently, ANAE+T ratio and T lymphopoiesis were increased by dietary supplemental copper.(5) Cu amino acid chelate increased mice's serum IgG content and monocyte phagocytosis, monocyte phagocytosis increased by dietary supplemental copper, and serum IgG content increased significantly.(6) Compared with 4.5mg/kg Cu treatment, 45mg/kg Cu supplemental level treatment showed no significant difference at nutrition and immune index.