| The random amplified polymorphic DNA (RAPD), was used in this paper to study the genetic diversity among four different serology Riemerella Anatipestifer (RA) and five pathogenic Escherichia coli and five salmonella from ducks. 8 primers were selected from twenty 10 bp arbitrary primers, and their amplification products were analyzed by cluster analysis. Then 1 primer was selected from above eight primers, which has authentication among interspecies, searching the characteristic bands.The results showed that:(1)These arbitrary primers could be divided into four groups, ï¹o amplification products, for example G-OK G-20; (2)no polymorphisms between amplification products, for example G-04, G-05, G-08, G-09, G-10, G-13, G-14, G-15, G-17, G-19; (Damplif ication products have enough polymorphisms, for example G-02, G-03, G-06, G-07, G-ll, G-12, G-16; ヾistinguish between interspecific, for example G~12.(2)The length of all amplification products are between 0.2- 3.2kb. the number of amplification bands are between 5-16. Totally 102 RAPD bands were identified, of which, 1 band were commom in all the accessions and 101 bands were polymorphic between any two accessions. The polymorphic bands accounted for 99% of the total amplified bands.(3)Cluster analysis were conducted for amplification products of eight primers, and the results accorded with traditional classification, that was the genetic distance matri between Escherichia coli and salmonella is closer, but the genetic distance matri between RA and Escherichia coli and salmonella is far.(4)The differential sign bands among RA and Escherichia coli and salmonella are 535bp, 1227bp and 330bp.(5)The differential sign bands were obtained when amplified with primer G-12 as mix-template.This search is first time to study polymorphisms among RA and Escherichia coli and salmonella by RAPD. The RAPD has the advantages of simple, quick, accurate and stable. |
PDF Full Text Request