The Research Of Mapping Maize Nuclear Sterile Gene Obtained By Space Flight With Simple Sequence Repeat

It is an important method of obtaining heterosis by using maize male sterility.The maize cytoplasm male sterile materials are infected easly by Corn southern leaf blight, so the search of maize male sterility was transferred from cytoplasm male sterile materials to nuclear male sterileity materials . We sent maize seeds for satellite aboard in 1996 and got a nuclear male sterile mutant in the offspring of the single hybrid-chuan Dan no.9 . hi order to analysis this material ,the sister cross population (RP3195 (A)xRP3195-l(B)) was detected with Simple Sequence Repeat moleculer marker .The main results concluded as follows.1 .There are seventy nine normal fertile plants and sixty nine male sterile plants in the population .the trait of male sterility was controlled by a single recessive gene in the chromosome.2.The analysis of Simple Sequence Repeat shows that no difference between normal fertile DNA bulk and male sterile DNA bulk was detected by 517 pair SSR primers, but 3 out of the 517 SSR primers took on polymorphsms when use single normal fertile plant and single male sterile plant DNA as PCR templation.3. When ampplyied these polymorphic markers with every plant in the segregating population ,only the microsatellite locus bnlglHl which mapped on chromosome 2L in maize took on co-segregating with the fertility expression of each plant in the sister cross population ,so this result suggested that the locus of bnlg1141 may be linked with the male sterile gene ms , according to the Mapmaker software analysis ,the genetic distance between bnlgl 141 and the male sterile gene ms was estimated to be 35.3cM .4.The strategy of touch down PCR was used in this research ,comparedwith the usually PCR ,it has a better speciality and amplication for each SSR primer,the reason why touch down PCR has a good result disscused in this article.