Effects Of Different Stress Factors On Metallothionein Synthesis In Mouse Liver

152 Kunming mice which were randomly assigned to 18 experiment groups and 1 control group with 8 replicas were used to study the effects of 5 stress factors and 19 treating ways on the metallothionein synthesis in liver of the mice. The levels of metallothionein synthesized in liver of the mice induced by different stress factors including trauma, starvation, zinc (Zn) nutriment, cadmium (Cd) poison, both Zn nutriment and Cd poison and different treating ways which were designed according to the time-effect and dosage-effect were detected by Atomic Absorb Mean and Sulfydryl Saturation Mean respectively. The results showed that:1 For normal acute toxicity of Cd, the LD50 of Cd intraperitoneally injected into mice was 2.116(2.670~1.7128)mg/kg .The mice were intraperitoneal injected Cd with the dosage of 1.00 mg/kg three days before acute toxicity of Cd, the LD50 of Cd for these mice by intraperitoneal injection increased from 2.116(2.670~ 1.7128)mg/kg to 2.537(2.9485?.1825)mg/kg.2 After treated with different Zn concentrations or Cd dosage, the levels of metallothionein synthesized in liver of the mice increased with the increase of Zn or Cd concentration. And there were significant (p<0.05) differences in the levels of metallothionein synthesized in liver of the mice between every two groupswhich were treated with different Zn concentrations or Cd dosage.The level of metallothionein in every experiment group treated with mixture of Zn and Cd was higher than that of the groups treated with the same Zn (Cd) dosage, but it was not the sum of the two groups .This proved that there be antagonism between Zn and Cd.The level of metallothionein in the group with Zn or Cd progressive increment was higher than that of the group with the same total Zn or Cd dosages. This suggested that Zn or Cd in animal body have progressive increment effect.3 The longer the mice starvation time was, the higher of the levels ofmetallothionein synthesized in liver of the mice were. But there were no significant (P>0.05) differences in the levels of metallothionein synthesized in liver of the mice between every two groups. Among all stress factors, the level of metallothionein induced by starvation was minimum.4 Among all stress, the level of metallothionein induced by trauma was the highest. The more serious the trauma was, the higher the level of metallothionein synthesized in liver of the mice was. And the longer the time of trauma recovery was , the lower the level of metallothionein synthesized in liver of the mice was . The quantity climax(9.0241?.6440#mol/g) of metallothionein came out among l~12h after trauma. There were significant (P<0.01) differences in the levels of metallothionein synthesized in liver of the mice between every two groups.