The Studies On Crown Gall Clutrue Of Panax Quinquefolium And The Production Of Its Active Constituents--Ginsenosides

The studies on the solid culture of Pan ax quinquefolim crown galls and the production of their secondary metabolite-saponins were reported in this paper.It was clearly demonstrated that the T-DNA in Ti plasmid of Agrobaclerium tumefaciens C58 was integrated into the nucleus of plant cell by the experiments of paper electrophoresis. The strain of crown galls with fast growth and genetic and biochemical stability were selected in different hormone-free media. The optimal culture conditions of growth and accumulation of saponins were achieved by using MS and White medium. The time course of crown gall growth and saponins accumulation were investigated, and the result showed that the growth and saponins content reached a maximum at 24th day and 28th day respectively. The effects of some physical and chemical factors on the growth and saponins content were examined. The compounds of sponins from cultivated crown gall were analysed qualitatively and quantitatively by means of TLC and HPLC, and the highest content of total saponion and ginsenoside Rb₁, Rgi and Re were 3.32%, 1.881%, 0.021% and 0.194% (DW), which were 1.52, 1.48, 0.21 and 1.94 folds compared withthose of natural plants respectively. The content of total saponion and ginsenoside Rb₁, Re were 1.28, 3.76 and 0.33 folds compared with those of calli cultures.