Constraction Of Recombinant Flowpoxvirus Expressing VP3 Gene Of Goose Parvovirus

Goose Parvovirus (GPV) is the pathogen of Goslings plague. This disease is highly fatal to goslings and muscovy ducklings under 1 month of age with the characteristics of acute enteritis and hepatonephric and myocardium lesions, having made large losses to goose breeding industry. In many countries the virus has been isolated after DingYi .F firstly found and isolated it in china, which suggested that this disease has been prevalent in the world.So far as, prevention of GPV infection mainly depended on vaccines. Although the traditional vaccines have played active role in prevention and controlling the disease, many deficiencies exist still such as inactivated vaccine being expensive and inducing strongly response at the injected tissue, as well as the recovery of the attenuated vaccine. The high conservative main antigenic protein VP3 of GPV provided the access to producing the new type vaccines and diagnostic agent. In this study, the recombinant fowl-poxvirus was transfected into expressing the VP3 gene of isolated GPV H1 strain into the CEF cells with FPV-017 by liposome, which have the LacZ reporter gene, earlier / latter promoters LP2EP2 of FPV, promoters P7.5 and P7.1 of Vaccinia virus, replication unnecessary region of FPV-017. Following 6 cycles screenings, clonings, purification of blue plaques, detection of PCR and Dot-ELISA, which verified the genetic stable VP3-fowlpox virus recombinant constructed successfully. This study provided the theoretical and practical foundation for development of GPV recombinant fowl-poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality GPV.