Accumulation Of LMW-GS And Its Effects On The Quality In Spring Wheat With Different HMW-GS Composition

The study aimed at the dynamic formation and accumulation of low molecular weight glutenin subunits, the relationship between low molecular weight glutenin subunits and quality, the dynamic formation and accumulation of soluble glutenin and macropolymer glutennin; the relationship between quality and the ratio of LMW-GS to soluble glutenin, the relationship between quality and the ratio of LMW-GS to macropolymer glutenin, the relationship between quality and the ratio of LMW-GS to HMW-GS, and the effects of different form of nitrogen on the accumulation of LMW-GS and the main grain quality parameters . The study aimed at providing theoretical evidences to quality improvement and high yield and superior quality in spring wheat.Three forms of nitrogen and 18 cultivars differing in HMW-GS composition were used. The results showed:The trend of accumulation of B, C and D group LMW-GS increased in grain-filling in different HMW-GS composition cultivars whereas the trend of accumulation of D group LMW-GS showed smooth. Besides NES18 and Quan224, amount of B group LMW-GS of all cultivars were higher than amount of C group LMW-GS in grain-filling in all cultivars. Effects of different form of nitrogen on LMW-GS were different in cultivars, but not types of HMW-GS composition.The effects of different forms of nitrogen on grain protein contents were different. The reactions of the same cultivars to three forms of nitrogen were different in two years. The reactions of grain protein content of NE7757 and Liao 10 to different form of nitrogen were unstable in two years, whereas the grain protein contents of NE7742, KF-6 and Roblin were consistent, and the reactions to different forms of nitrogen were stable in two years. In two years, the wet gluten content of NE7757, KF-6, NKH9and keepawa were the highest under Urea treatment respectively, while NES18 and NES250 were the highest under NH4+-N treatment respectively, and the reactions of the wet gluten contents of poscin to three forms of nitrogen were stable in two years. The reactions of other cultivars to forms of nitrogen were different.The effects of different forms of nitrogen on grain wet gluten contents were different in cultivars with different HMW-GS composition. To cultivars with 2+12 subunits composition, Urea treatment were good to increasing wet gluten contents of NE7757, KF-6 and NKH9, and the effects were stable in two years; NH4+-N treatment was good to increasing wet gluten contents of NES18 and NES250; NO3"-N treatment was good to wet gluten contents of NES57, and the effects were stable in two years. To cultivars with 5+10 subunits composition, effects of three form of nitrogen on wet gluten contents of poscin, LF-3 and LM 26 had no significant different, and the trends were similar in two years.The reactions of SDS-sedimentation value of the same cultivars to different forms of nitrogen were different among cultivars. Urea treatment was good to increasing SDS-sedimentation value of NES250, poscin, NECV8542 and NE S34, the values were the highest among three forms of nitrogen, and the effects were stable in two years; NO3-N treatment was good to increasing SDS-sedimentation value of NES18 and NH4+-N treatment was good to increasing the SDS-sedimentation value of LM26, and the effects were stable in two years too. Effects of three forms nitrogen on SDS-sedimentation value of LiaolO were similar, and the effects were quite stable in two years, especially Urea treatment.The results showed: the quality of the cultivars with 2+12 subunits composition were good, their final accumulation amount of macraopolymer glutenin were much higher than the amount of cultivars with 5+10 subunits'composition, and their final accumulation amount of soluble glutenin were higher than the amount of cultivars with 5+10 subunits composition, at least the two had similar amount.Effects of the ratio of total amount of LMW-GS and total amount of soluble gluten on quality were different in three forms of nitrogen in cultivars with different HMW-GS subunits compos...