| Three experiments were conducted to study protease and amylase in stomach,intestine and hepatopancreas of Silurus asotus linnaeus. The results are followed:Experiment one: The experiments were conducted to study the pH value and the digestive tube index in stomach,intestine,hepatopancreas of Silurus asotus linnaeus, The results are followed:1. The pH value in digestive tube of Silurus asotus linnaeus is changed from stomach to post intestine.The pH value of stomach is 4.8-6.2,and that of post intestine is 6.5-7.0.the acidity of stomach is very strong . Not only it can acidulate the feeds,but also can be benefit to the effect of protease in stomach.2. The value of intestine length / body length of Silurus asotus linnaeus is 0.726+ 0.061,the stomach weight / body weight and hepatopancreas weight / body weight are 0.027 ± 0.006,0.022 ± 0.0029 respectively.it is smaller than that of herbivorous fishes and omnivorous fishes. The ratio of stomach,intestine, hepatopancreas and viscera weight to body weght of Silurus asotus linnaeus are deduce with growing.Experiment two:With Silurus asotus linnaeus, The activity , optimum pH and optimum temperature of protease and amylase in shomach,intestine and hepatopancreas were measured. The results are followed:1. The protease activities of stomach, antio intestine,middle intestine,post intestine and hepatopancreas are 152.27±3.53,254.55±2.74 ug/g.mm,75.55±2.69 ug/g.min,64.59±3.16 ug/g.min and 179.08 ±1.97ug/g.min in Silurus asotus linnaeus respectively .The amylase activities are 242.05±4.24 mg/g.h, 196.67±1.59 mg/g.h, 166.41 ± 1.06 mg/g.h, 151.28±2.25 mg/g.h and 1013.58 ± 4.83mg/g.h respectively.the difference was significent (P<0.05) .2. The optimum temperature value of stomach, intestine and hepatopancreas for protease are40℃, 44℃ and 36℃ respectively ,and for amylase are 44℃,40℃ and40℃ respectively in Silurus asotus linnaeus.3. The optimun pH value of shomach, intestine and hepatopancreas for protease are 2.2,7.0 and 7.4 respectively,and for amylase are 7.0,7.4 and 7.0 respectively in Silurus asotus linnaeus,Experiment three: The experiments were conducted to study enzymolysis kinetics and digestive rate in vitro of Silurus asotus linnaeus to fish meal,soybean meal,rapeseed meal, cottonseed meal, meat meal, brewers dried yeast and beer gluten meal.The enzyme is crude enzyme that was obtained from stomach, intestine and hepatopancreas in the fish. The digestive ability was determined:1. The digestive rate of fish meal,soybean meal,rapeseed meal, cottonseed meal, meat meal, brewers dried yeast and beer gluten meal to the dry matter are 49.15%, 44.98%, 41.44%, 47.37%, 46.68%, 35.32% and 31.73% respectively;those of the crude protein digestibility are 49.93%, 48.15%, 39.44%, 28.34%, 34.50%, 24.16% and 20.94% respectively(P<0.05).2. The rates of amino acid were producted from the enzymolysis fluid of fish meal,soy bean meal,rapeseed meal, cottonseed meal, meat meal, brewers dried yeast and beer gluten meal are 16.45Img/h, 14.756mg/h, 14.114mg/h, 11.836mg/h, 13.177mg/h, 11.429 mg/h, 10.674mg/h respectively (P<0.05 ).The result indicated that:1. The digestive ability is adapt to feeding and construction of digestive tube.the digestive tube index are consistent the other garnivoroue fishes.the digestive ability to protein in feeding is very strong.2. The activities of protease and amylase in different part of gut were different.the activity of protease in antio intestine is the hightest,that of hepatopanceas is the second, that of middle intestine and post intestine are low; the activity of amylase in hepatopanceas is the hightest,that of stomach is the second,that of middle intestine and post intestine are low within 0-4 hours. The protease activity in the hepatopancreas is the hightest,and that in the post intestine is lowest.the amylase in the hepatopancreas is higher than that in the intestine and stomach(P<0.05).The front digestive tube of Silurus asotus linn... |
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