Studies On The Characteration And Immunogenicity Of Different Serotypes Of Outer Membrane Protein Of Riemerella Anatipestifer

Four major serotypes of Riemerella anatipestifer-serotype 1,2,4,5 are chosed for the test, we extract their outer membrane protein and study their immunogenicity.Ultracentrifugationg-Sarkosyl extraction was chosen for the isolation of the outer membrane proteins (OMP) of Riemerella antipestifer (RA). Under the electron microscopy, the ultra structure of RA was observed. The OMP of RA showed the typical double round vesicules with negative stain under electronic microscopy; however, there are some differences among the morphologic. Some are O form, some are tooth form.The electrophoretogram of the four serotypes is some similarity after SDS-PAGE, but there are many differences among them. The molecular weight of OMP of serotype 1 RA ranges from 25kd to 143kd, among them, 31KD,44KD,69KD,77KD,83KD, 91KD and 107KD proteins are more heavily stained by Commassie blue; serotype 2 RA ranges from 26kd to 135kd, among them, 31kd,33kd,75kd, 101kd and 116kd are more heavily stained by Commassie blue; serotype 4 RA ranges from 26kd to 124kd, among them, 32kd,28kd,80kd,114kd are more heavily stained by Commassie blue; serotype 5 ranges from 28kd to 121kd,among them, 31kd, 43kd,70kd,89kd,92kd,lllkd are more heavily stained by Commassie blue.The 44KD of OMP of serotype 1 RA have strong masculine with duck serum reaction by WESTERN-BLOTTING; the 40KD of OMP of serotype 2 RA appears obvious reaction with duck serum by WESTERN-BLOTTING; the 75KD of OMP of serotype 4 RA appears obvious reaction with duck serum by WESTERN-BLOTTING; the 39KD of OMP of serotype 5 RA appears obvious reaction with duck serum by WESTERN-BLOTTING;From the result of duckling immured by outer membrane protein, we know that OMP of all the four serotypes can induce obvious antibody reaction, and the protective ratio of the attack from the RA of the same bacteria was 100%.