Expression Of Hepatitis C Virus Structural Proteins In Insect Cell Using The Baculovirus Expression System

Containing many T cell epi-positions and neutralizing antibody epi-positions, the core protein region and envelope protein 2 of Hepatitis C virus (HCV) structural protein may induce protective immunity responsion in body. In this experiment HCV structural gene was amplified by polymerase chain reaction (PCR), and was inserted into baculovirus expression vector pFastBacl to construct a recombinant transposing vector pFBl-CEE. The plasmid pFB 1 -CEE was transformed into DH1 OBac competent E. coli cells. High molecular weight DNA was prepared from the overnight cultures from the selected E. coli colonies, which was recombinant baculovirus shuttle vector containing HCV structural gene, named Bac-CEE. Spodoptera fragiperda (Sf9) insect cells were transfected with the recombinant Bac-CEE via lipofectin. Fresh insect Sf 9 cells were infected with the recombinant virus to express the target protein. The structural protein recovering from the Sf 9 cells exhibited protein stripe. The expressing HCV structural protein in this experiment will make further development for HCV preventive vaccine study.Postgraduate: Fei Li Major: Microbiology and ImmunologyTutor: Prof. Jingpeng Li...