| In recent years, embryo transfer technology has made great contributions to increasing the amount of goats for breeding in a short period, but the success rate of embryo transfer was not stable in practice. Many people from native or abroad have carried out lots of study on improving the rate of success, but most of them discussed only about one factor affecting the rate of success. A comprehensive study on the influencial factors in the course of embryo transfer will help to improve the rate of success and enhance the stability of it. In this study, 30 Boer goats as donor; 89 LUBei white goats as recipients of estrus synchronization, analysis on varieties of factors affecting the superovulation, synchronization and the rate of pregnancy.Analysis on 5 kinds of targets for superovulation by normal test showed: CL (corpus luteum) number, embryo number, usable embryo number were normal, while metamorphic embryo number and no zygote number were unnormal. So nonparametric test method should be adopted for the later; Multivariate Analysis on 6 kinds of influential factors for the superovulation effect showed: The injection time of FSH and estrous synchronization methods were two main reasons to take the different effects of superovulation among all the factors. The intercept effect was not significant.Analysis on 6 kinds of influential factors for superovulation effect with One-way ANOVA Analysis and Nonparametric Test. The result showed : The CL number of the MM (more than middle level of body status ) and M(middle level of body status ) groups were more than other groups significantly (11.17?.29 and 6.22?.37).It showed that the main effect that defferent body status affected the superovulation was the CL number; The effect of superovulation of the 7th or 9th day in the estrous period was better among different injection time of FSH. It showed that the ovum vesicle growth wave of Boer Goats should begins with the 7~9th day in the estrous period. CL numbers were different significantly in the different method for synchronization (PO.01). PG 2ml (2 mL /once) was injected to donor, which affected the rate of fertilization and usable embryo number significantly. There were no difference between the bolt group and the injection group. The result showed that PG 2 mL (interval 12h, 1 mL /once) was injected, which can produce perfect effect of superovulation and estrous synchronization; The rate of picking embryo (29.56%<63.58%) and the rate of usable embryo (75.30%<94.34%) in the injection group with LH were lower than no injection group, and the rate of no zygote (20.07%>1.37%)was higher than no injection group significantly. It showed that LH was injected to the donor, which increased the number of ovulation of premature follicular; The embryo number(6.29 ?1.57)and usable embryo number(5.10?1.56)in the 24 h estrus group (donor's estrous time was in the 24h after estrous synchronization)after synchronization were higher than the 36 h or 42 h estrous group, but the rate of no zygote (15.90%) was higher than other two groups.The difference for the effect of superovulation among three groups was no significant (P>0.05). It showed that the estrous time of donor couldn't affect the effect of superovulation significantly; Mating assistance with AI can reduce significantly the no zygote rate (PO.05), morever improve significantly effect ofsuperovulation.The rate of synchronization within 36 h of donor was 100% in the PG group. PG was injected 2 mL (interval 12 h , 1 mL /once )can make the rate of synchronization within 24 h reach to 80%; The correlated coefficient between the CL number and embryo number was 0.415(P<0.05), the correlated coefficient between the embryo number and usable embryo number was 0.928(P<0.01), we can predict the embryo number and usable embryo number through the CL number .Four factors affecting the rate of recipient were discussed with Logistic Regression Model. The result showed that the method of transfer and the status of estrous affected the rate of recipient pregnancy signifi... |
