| 90 materials of Hebei province soybean including 83 local cultivars and 7 breed cultivars were studied using random amplified polymorphic DNA(RAPD) and numerical taxonomy method. The aim of this study was identifying Hebei province soybean cultivars by RAPD pattern, putting forward the taxonomy system of soybean cultivars based on knowing the genetic relationships of Hebei province soybean groups, and trying to establish core collection. The main results were as follows:1. A extraction method from dry soybean seed for genetic DNA isolation of soybean was set up. The extraction from dry seed not only can save many times but also can cut down expenses, in addition, the RAPD analysis results were fairly good.2. RAPD analysis technical system for soybean was established by studying different factors that might affect RAPD results. 46 primers were selected to be highly polymorphic primers from 84 arbitrary 10-mer primers. 30 primers were used in RAPD analysis of 90 materials, and a total of 209 DNA bands were amplified, among which 159 (76.08%) were polymorphic. Different RAPD patterns of 90 soybean materials had obtained with different primers. At the same time, Different RAPD patterns of one primer had obtained with different soybean materials.3. The numerical taxonomy of 90 Hebei soybean materials was studied by clustering analysis of RAPD. The relationships of Hebei province localcultivars and breed cultivars were discussed. The cluster analysis indicated that breed cultivars clustered together and the genetic distances between them and local cultivars were longest. The cluster results of all the materials coincided with geographic origin.4. The method of establishing Hebei province soybean core collection was studied preliminarily at molecular level. Based on the genetic relationships of 90 Hebei province soybean materials, the core collection containing 32 basic cultivars had been established for the first time. A model to establish soybean core collection had been put forward.
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