Study On Genetic Transformation Of Anti-Apoptosis Gene CED-9 To Tobacco And Rice

Plant programmed cell death (PCD) is increasingly becoming the focus of intensive research. In this paper, we transferred CED-9 gene, an anti-apoptosis gene, into tobacco and rice by Agrobacterium tumefaciens EHA105. The results are showed as follows:1. Using recombination techniques, the gene fragment CED-9, derived from pBS, was incorporated into pGEM-7zf(+) and formed a new vector pGEM-ced9, in order to get the right endonuclease sites. And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. The CED-9 gene was transformed into leaves of tobacco by Agrobacterium tumefaciens EHA105. After twice selections of kanamycin cultures, 96 plants were regenerated from selective medium, and about 34% of them turned out to be transgenic. PCR analysis and Southern blotting all confirmed that the CED-9 gene has been integrated into the chromosomal DNA of these transgenic plants.2. We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. Then we constructed the expression vector pCA-ced9, and transferred CED-9 gene into the rice callus and embryogenic suspension cells. The work of using hygromycin selective medium to obtain regenerated plants is still going.