Studies On Genomic Variance Among Anhua Yun-tai-shan Populations Of Tea Plants

Random amplified polymorphic DNA (RAPD) has been used in analyzing twenty seven tea plants of Anhua Yun-tai-shan and Xiangan No. 28,Zhuye-qi and Xiangbcrlu . The results showed that the average yield of the DNA by the method was 425.4 ug/g ?FW, and the length of the DNA fragment was more than 21Kb. The isolated DNA could be directly used for RAPD analyses . By using 120 random 10-mer primers, RAPD polymorphism were detected in 30 samples. The polymorphism information could be provided with 21 random primers. Total 150 loci were amplified, 119 loci of these were polymorphic (79. 3%). Each primer gave 3-10 bands and the average was 7. For 21 arbitrary primers, each individual showed about 82 RAPD markers. Some distinctive bands were found in each sample. Agarose electrophoresis showed that abundant genetic variations existed in the populations. The genetic distance (D) of twenty seven tea plants of Anhua Yun-tai-shan between YTS9 and YTS12 is 0. 1252, which is the lowest. The highest D Value is 0.3939, which is between YTS6 and YTS30. The genetic distances showed that considerable genetic variance and clear genetic divergences existed in the populations. According to the UPGMA analysis , Xiangan 28, YTS31 and Zhuye-qi were gathered to a branch in the dendrogram, and Xiangbo-lu was clustered preceding YTS30. It had been proved on the DNA molecular level, Xiangan 28, Zhuye-qi and Xiangbo-lu originated from Anhua Yun-tai-shan populations. The results also showed that it is feasible to select new varieties from tea plants populations by systematic seed seletion. The RAPD can identify the genetic variance and genetic relationships between parents availably, and provide sufficient evidence for a feasible mean in the choice of parents for the development of tea plants and hybrids.