| Avian influenza is an infection and / or disease syndrome caused by any subtype of influenza A virus, a number of the Orthomyxoviridae family. Influenza A viruses are responsible for major disease problem in bird, as well as in humans and lower mammals. Literally thousands of viruses, belong to many different antigeAubtypes based on hemagglutinin(HA) and numatninidae(NA) surface antigens, have been recovered from domestic and wild avian species throughout the world. Infection among domestic or canfined birds have been associated with a variety of disease syndromes ranging from subclinical to mild upper respiratory disease, loss of egg production to acute generalized fatal disease. Influenza A virus are divided into subtype according to the antigen nature of the HA and NA. So far there are 15 subtype of antigen of I-IA and 9 subtype of NA antigen, in which avian influenza viruses of H5 and H7 subtypes have been associated with severe disease in chicken, turkey, domestic duck and tern. The highly pathogenic isolates have caused severe disease with morbidity and mortality reaching IOO0/c. With the development of economics and exchange of poultry and its products, avian influenza has occurred in Southeneast China. And a few highly pathogenic influenza viruses were isolated from goose and duck. In this paper, the diagnose prevention and cure of avian influenza were studied on according to avian influenza actuality in our country. In expriment 1 The eDNA of M2 gene of Avian influenza isolate AlGooselGuangDong/l/96 (HSNI) was amplified by RT-PCR and subsequently cloned into pMDI8-T Vector. Restricted enzyme analysis and PCR analysis confinned the presentation of the M2 cDNA in recombinant plasmid. Then recombinant plasmids were sequenced. The result of sequencing showed that 31 lbp of M2 cDNA covered the whole M2 gene including the complete open frame 297 bp, encoding 97 amino acids resides. In expriment 2 Matrix-encoding eDNA of an avian influenza virus (AIV) GD/1/96{H5NI) was subcloned into pFastBacl vector from pUCMI plasmid. The presentation of the Ml eDNA in recombinant plasmid was confirmed by PCR analysis. Recombinant bacmid (rBacM) was obtained by transform plasrnid pBM into DHI0bac cells. After confirmed by PCR. the rBacM was trans-infected into sf9 cells packed with CeIIFECTIN regent. SDS-PAGF and Western blotting determined the expression of M gene in sf9 cells. The results showed that the expressed product had a molecular mass of approximate 27K.D, accounting for 8.9% approximately in whole cellar proteins. The AGP test showed recombinant Ml protein had potential of diagnose antigen. In expriment 3 According to reproduce characteristic and mechanism of soliciting disease of avian influenza virus (AIV). we developed new-style drugs 慔UDULIN. The control effect of new-style drugs 慔UDIJLIN?on avian influenza was studied on in this paper. The 96 chickens, which were 30 days old, were randomly divided into 5 groups. Group I wasn抰 inoculated MV and given drugs; Group 6 was used intravenously (i.v.) by 0.2niI chicken embryo urine per chicken which didn抰 infect MV. Group 2, 3, 4, 5 were i.v. inoculated MV ( 0.2m1 per chick). Group S serve as control, the others were given drugs 1. 2. 3 before inoculating MV. T lymphocyte tranformation activity was tested at the 4th day afte inoculating AIV by H3桾dr incorportion assay with lymphocyte sepration sepration culture.The 30 AA chickens were divided into 3 groups, which were used to do security experiment of drug~ 1.. 2.. 3. After inoculated, the chickens were observed every day. The obituary chicken were pathologic dissection. The results...
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