| 428,492,492bp DNA fragments are amplified by PCR with the degenerated primers designed according to the mariner sequence of Drosophila mauritiana, the genomic DNA of Nilaparvata Lugens, Sogatella furczfera and Laodelphax striatellus as the templates. The sequences have been gained after recollecting .~ cloning and sequencing. Sequence analysis shows that the DNA similarity of Nilaparvata Lugens, Sogatella furcjfera and Laodelphax striatellus to that of Drosophila maurztiana is 37.0%, 69.2% and 71.3% respectively. This indicates that the mariner element may exits in Nilaparvata Lugens, Sogatella furc~fera and Laodelphax striatellus, which provides a possible approach for the study on the genetic transformation of planthoppers and other insects. About 500 bp fragments can also be amplified by RT-PCR, the total RNAs of the three planthoppers are as the templates. It indicates that the mariner gene may be an active transposable element. Three anchored primers are designed for 3?RACE. Several fragments are obtained with each primer, which indicates the mariner gene may be active. Symbiotes are isolated and purified from Nilaparvata Lugens ~. Sogatella furczfera and Laodelphax striafellus. The genomic DNAs are extracted for dot hybridization. The results indicate that the mariner gene obtained stems from the three kinds of planthoppers, not from the symbiotes. |
