Salt-related Genes In Populus Euphratica

(Summary)Salt-related Genes in Populus euphratica*Zeng Huiming (Forest Tree Breeding)Directed by: professor Xiang Yuanyin, associate professor Shen Xinand Bai GengbenPopulus euphratica Olive. is distributed across arid and saline areas in northwestern China and the predominant species of forestry in these areas. It is very important to sustain the ecosystems while promoting economic development in these areas.In order to analyze the molecular mechanism for salt tolerance in P Euphratica, salt-related genes were screened through Suppressed Subtractive Hybridization (SSH) with the results as follows:1.Subtractive hybridization showed that some special genes were inducibly expressed under salt stress with 119mM NaC1 solution for one hour in tender roots of two-year-old P euphratica cultured in sand.2.Labelled ci -32P-dCTP, two probes of salt-induced genes and one probe of salt-suppressed gene have been screened out through six cDNA array inspects with 672 clones.3.One of the salt-induced genes has been sequenced by means of Rapid Amplification of cDNA Ends (RACE) and found to contain an Open Reading Frame (ORF) encoding a polypeptide of 188 amino acids, which had been previously identified.4.The gene has been registered in GenBank, the accession number was AF3 15118. Homologue checked in GenBank with BLAST suggested that the salt-induced gene worked for detoxification and protection of molecular and cellular structure.