Transformation Of Muskmelon With Amaranthum Caudatus Agglutinin (ACA) Gene And Studies On The Gene Expressing Of Transgenic Plants

Plant expressing vector pBCACA was constructed containing a potential aphid resistant gene ACA. ACA gene in pBCACA was constructed so that its expressing in plants would be under the control of Commelina Yellow Mottle Virus (CoYMV) promoter and It was transferred into muskmelon by Agrobacterium ?mediated transformation method. Some kanamycin resistant plantlets have been regenerated. Results of PCR indicated that ACA gene was probably integrated into the genomes of muskmelon plants.A set of complete technique of muskmelon was constructed. (1) When preparing sterile seedlings of muskmelon, seeds were sterilized directly and the seeds coats were not removed. HgCI2 method is more suitable for muskmelon than NaOCl method, when comparing the two methods. (2) During the transformation and regeneration of muskmelon, the density of 6-BA is I .Omg/L is suitable for this muskmelon species ; cotyledon is a good explant for muskmelon transformation and regeneration plantlets can not be acquired when using hypocotyl as explant ; a lot of embryo callus was acquired when selecting gray-white and light yellow callus to regeneration and thick green callus with smooth surface can not produce embryo in the later time.In order to get a quick understanding of the function of ACA gene in insect resistance in plants, ACA gene was transformed into tobacco plant simultaneously. Results from PCR and Weston blot analysis of transformed tobacco plants indicated that ACA gene has probably been integrated into the genomes of tested plants and efficiently expressed in transformed tobacco plants. The results of insect bioassay with peach aphid (Myzus persicae) showed that the transformed plants were aphid ?resistant evidenced by a 6 5.65% reduction in aphid population density.