Molecular Tagging Of Ms2, A Dominant Gene For Male Sterility In Common Wheat (Triticum Aestivum L.)

The Taigu Genie Maleterile Wheat and Dwarfingterile Wheat are unique genetic resources in China. Experience in wheat breeding has proved that they are effective for various breeding procedures. Therefore, it is of significant importance to study the molecular basis of the genie maleterile materials. It will further broaden its range of utilization, and raise its use efficiency.In the present study, a series of backcrossing populations in which Chinese Spring was used as the recurrent parent, and Taigu Genie Maleterile Wheat and Dwarfingterile Wheat which carry maleterile gene Ms2as the donor parents, are determined. The molecular markers linked to the maleterile gene Ms2 was screened using AFLP and SSR techniques, and the genetic similarity between the backcrossing progenies and recurrent parent was detected. The main results were as follows:The amplified fragments length polymorphism(AFLP) analysis was conducted in Ms2 nearsogenie lines(NILs), with 669 pairs of randomly selected primers, using two restriction enzymes MseI and SseJ, and silvertaining in AFLP technique. Fortyhree out of the 669 pairs of tested primers amplified the polymorphic DNA in the NILs. One pair of primers(MCC/PGA) of them showed the same discriminating result in many replications. The polymorphic band associated with Ms2 is about 500bp, and assigned to be AF500. Further AFLP analysis of the segregated populations of NILs by primers MCC/PGA with Joinmap 1. 4 , indicated that the genetic distance between AF500 and Ms2 was 18.0?.0cM.Through the analysis of backcrossing materials using 1003pairs of AFLP primers, average 50 clear bands each pair of primerswere detected, for seven out of the l00 pairs of tested primersamp1ified the po1ymorphic DNA in the NILs. The statistica1ana1ysis showed the genetic simi 1arity between the recurrentparent and ferti1e p1ant is 99. 84%, and sterile p1ant ofbackcrossing progenies is 99. 78%, fert i1e p1ant and steri 1e p1antis 99. 82%. These results suggested that the great majority ofgenetic background of the backcrossing progenies were from therecurrent parent, that is to say, these backcrossing materia1s aresimi1ar to the recurrent parent in genotype except a sma1l regioncontacting target genes.There was no microsate11 ite markers 1inked to ma1e--steri 1egene M using 9 pairs of SSR primers of known posi tion on the armof chromosome (4D) where the nd gene located, and l00 pairs of SSRprimers of unknown position.