| Double factor experiment design was used with two pe NDF levels and two NFC/NDF levels(2×2).Diet NFC/NDF levels were 0.63 and 0.80, pe NDF levels were 49.85% and 53.64% respectively. Twenty wether sheep were randomly allocated into four treatments with 5 replicates in each treatment. Effects of different combination of NFC/NDF and pe NDF on chewing activity, rumen fermentation, blood biochemical indexes, nutrients apparent digestibility were determined.The results showed: pe NDF had(1)the significant effect(P<0.05) on intake, rumination and chew time of sheep. Dietary NFC/NDF had no significant effect on intake time, rumination and chew time of sheep(P>0.05). For the intake time, rumination, chewing time, high level pe NDF diet group was significantly higher than the low pe NDF level diet group(P<0.05); the high NFC/NDF diet group was lower than low level NFC/NDF level diet group, but the difference was not significant(P>0.05). Different pe NDF and NFC/NDF levels had no significant effect on intake, rumination, and the total chewing time of sheep(P>0.05). When feeding diets of pe NDF was 53.64% and NFC/NDF was 0.63,the intake, ruminaion, and chewing time of sheep were the longest.(2) The rumen p H, ammonia nitrogen, acetic acid, propionic acid, acetic-propionic, butyric acid were different significantly by different pe NDF levels in dietary(P<0.05), and the rumen p H, acetic acid, propionic acid, acetic-propionic, butyric acid were different significantly by different NFC/NDF levels(P<0.05),but no significant effects on bacterial protein(P>0.05). High level pe NDF treatments were significant higher than the low pe NDF level diet treatments for rumen p H, ammonia nitrogen, acetic acid, acetic-propionic(P<0.05), and the high pe NDF level treatments were significantly less than low pe NDF level diet for propionic acid and butyric acid. High NFC/NDF treatments were significantly less than low NFC/NDF diet groups for rumen p H, acetic acid, acetic-propionic and butyric acid(P<0.05). High NFC/NDF level groups were significantly higher than low NFC/NDF diet groups for propionic acid(P<0.05). The rumen average of p H, acetic acid, and the acetic acid/propionic acid ratio in group 2 which combination diet of pe NDF 53.64% and NFC/NDF 0.63 were higher significantly than other groups.(3) There were no significant effects on blood biochemical indexes in different pe NDF and NFC/NDF levels(P>0.05).,The total protein, albumin, glucose content in blood of the third group sheep which feeding high level NFC/NDF(0.80) and the low level pe NDF(49.85%) dietary were higher than other groups.(4) The DM digestibility was decreased significantly in pe NDF levels treatment(P<0.05).The DM, OM apparent digestibility were increased significantly(P<0.05), the NDF and ADF digestibility were decreased significantly(P<0.05) in NFC/NDF level diets. There were no significant interactionon apparent digestibility of nutrients in different pe NDF and NFC/NDF level(P>0.05). The apparent digestibility of DM, OM, CP, the contents of nitrogen intake, fecal nitrogen, retention nitrogen, and the total nitrogen utilization were decreased were accompanied by pe NDF level increasing, however, the apparent digestibility of NDF, ADF, and fecal nitrogen/nitrogen intake, urea nitrogen/nitrogen intake were increased. With NFC/NDF levels increasing, the apparent digestibility of DM, OM, CP, and contents of nitrogen intake, fecal nitrogen, urine nitrogen, retention nitrogen, and total nitrogen utilization rate were increased, and the apparent digestibility of NDF, ADF, fecal nitrogen/nitrogen intake, urinary nitrogen/nitrogen intake were decreased. Take together, the nutrient digestibility and utilization rate of sheep was higher than other groups when the dietary NFC/NDF was 0.80 and pe NDF was 49.85%. |
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