| Interferon has broad-spectrum antiviral and immunomod μ Latory activity and plays an important role in the body’s resistance to exogenous viral pathogen infections. At pre sent, natural interferon rIFN as well as interferon which from recombination in prokaryo tic or eukaryotic expression has been applied in clinical treatments. E. coli recombinant porcine interferon soluble expression of interferon expressed a broad spectrum of antivi ral activity, eplicting outstanding ability of anti-virus in a wide variety of animal-deri ved cells. The experiments were conducted by using an animal model of infection of ce lls and efficacy of recombinant interferon test for porcine transmissible gastroenteritis,ma king the following progresses:1 Inhibition test of recombinant interferon in PK15 cells by applying a animal model t est the role of cells infected with a for TGEV.Different dilution interferon pretreatment PK15 cells and then TGEV infected cells has been applied. Some res μ Lts were gaine d that in the 32-fold dilution induced cytopathic TGEV was completely inhibited by st andard interferon which showed consistent antiviral capability with human interferon. It was also found by the recombinant infection addicted prime spot in the 32-fold dilutio n completely inhibited the formation of plaques TGEV viral tropism while in the 64-fo Id dilution, formation of plaques TGEV viral tropism was significantly inhibited. In addi tion semi-quantitative RT-PCR and Western-Blot were applied to measure the expression from pretreated cells infected with TGEV virus nucleic acid and protein It revealed tha t the expression of the viral genome replication and TGEV specific proteins in cells ca n significantly be inhibited by recombinant interferon, which has higher efficiency than standard interferon.2 Weaned piglets were infected with diffenrent dose of TGEV vir μ Lent strain SC-T. All infected piglets showed typical clinical symptoms and co μ Ld shedding virus though feces. Pathlogical analysis displayed that middle and high dose s of inoc u Lation piglets showed characteristic pathological changes,which indicated that TGEV ir μ Lent strain SC-T co μ Ld susccessf μ Lly establish animal infection model. B ased on the infection model, piglets inocated with TGEV were injected with different d ose of recombinant porcine inferferon a. The res μ Lts shewed that the groups injected with 2 x 104 or 2×105 IU recombinant porcine inferferon a co μ Led cure 80 of pig1 ets infected with lethal dose of TGEV. In addition, piglets infected with lethal dose of TGEV were injected with recombinant porcine inferferon α at 0,24 and 48 hours post i nfection. The res μ Lts showed that at 0,24 and 48 hours post infection co μ Ld acquire 100%,80% and 70% recovery rate respectively. Three batchs of recombinant porcine i nferferon a were used and all batches showed the same antiviral activity.Those res μ Lts showed that recombinat porcine interferon a have favourable anti-T GEV activity both in vivo and in vitro.Using recombinat porcine interferon a at the ear ly stage of infection co μ Ld signally reduce the morthality of pielets which indicated th at the recombinant porcine inferferon a has favorable clinical application.
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