Analysis Of Mycorrhizal Protein Of Amorpha Fruticosa Using ITRAQ Technique

Taking arbuscular mycorrhiza fungi (AMF)-Amorpha fruticosa symbionts as the experimental materials, this study, during the symbiosis process between AMF and A. fruticosa, found that AMF had a significant promoting effect on A. fruticosa growth, according to fungal colonization percentage and plant growth status. And this promoting effect displayed more obvious with increase in intensity of fungal colonization. The late stage of the symbiosis formation, as the mature and most representative stage, was selected and isobaric tags for relative and absolute quantitation (iTRAQ) technology combing with two-dimensional liquid tandem mass spectrometry was employed to investigate the expression of A. fruticosa mycorrhizal proteins at the maturation stage (including symbiotic period of signal recognition and maturity) through proteomic analysis.The results showed that totally 3473 proteins were identified, of which 77 root proteins changed dramatically in expression, with 33 proteins up-regulated,44 down-regulated, and besides,9 AMF proteins were identified. Subsequently, the successfully identified proteins were classified according to their functions. Plant proteins were assigned into eleven categories including metabolism related(32%); protein folding and degradation related (22%); energy related(10%); protein synthesis related(8%); stress and defense related(24%); transcription related (6%); membrane and transport related(4%); cellular structure related(2.5%); signaling transduction related(11%) and unknown proteins (5%); on the other hand, the fungal proteins were assigned into five categories, they were:metabolism related(34%); protein folding and degradation related (22%); cellular structure related (22%); energy related(11%); protein synthesis related(11%). These implied proteins that related to plant metabolism, plant protein folding and degradation and energy, and proteins that related to fungal metabolism, protein folding and degradation, and fungal cell structure, played an important role during the symbiosis process of AMF-A. fruticosa, and at the same time verified that the symbiosis formation between AMF and A. fruticosa was the uniform and harmonious results of every process. The results of the study will provide theoretical basis for further revealing the metabolic characteristics and molecular mechanisms of arbuscular mycorrhiza (arbuscular mycorrhiza, AM).