Mixed Culture On The Impact Of Growth And Hydrogen Production Of R.palustris And Ecology

Hydrogen become the most promising alternative energy sources because of its renewable, clean and efficient and other prominent advantages. Biological hydrogen production aroused people's concern in recent years because of energy conservation and not consumption of fossil resources. The mixed culture of photosynthetic bacteria as an important direction for the development of hydrogen energy, since mixed culture of photosynthetic bacteria can improve the substrate and energy use efficiency of groups and increase production of hydrogen.The article includes photosynthetic bacteria on hydrogen production, different types of nutrition and performance strains will be formed into a mixed ecological groups which is highly efficient and three-dimensional, which can improve the effective use of substrate and energy and increase the production of hydrogen, R. palustris and different types of nutrition bacteria will be mixed for energy metabolism, growth, production of hydrogen, carbon metabolism and other aspects of research.The following research is:①Optimization of R. palustris culture conditions.②Hydrogen production and ecology of R. palustris and non-purple sulfur bacteria mixed culture.③Hydrogen production and ecology of R. palustris and anaerobic ferment bacteria mixed culture; At the same time use the advanced Biolog carbon metabolism plate methods, analysis the differences in growth of R. palustris pure bacteria and mixed culture from the perspective of growth and metabolism. The results show that:①the best growth and hydrogen-producing results of R. palustris is under the mode of pH 6.5~7.0, the light intensity for the 5000 ~ 6000 Lx, temperature for 30~35℃and anaerobic light conditions; the promotion factor of bacterial growth plays a significant role in the growth of bacteria, the promotion factor includes pyruvic acid sodium and catalase.②Bacteria can exchange of information through quorum sensing system, emerging signs of movement and gathering, some colonies exist in depression in use of the near-natural pure culture technique cultivate.③R. palustris was identificated by Biolog carbon metabolism plate ,which will be contrast to the metabolism of mixed bacterial.④The best metabolism model of R. palustris and R. sphaeroide mixed culture for the anaerobic light conditions.There have some mutual competition and inhibit before 24 h; The maximum hydrogen production of R. palustris and E. aerogenes PCM 532 mixed culture amounted to 128 mL. Anaerobic fermentation bacteria have great support role on the hydrogen-producing of photosynthetic bacteria.⑤To the growth of bacteria cell promoting factor play a certain role, There have the greatest impact on growth of mixed bacteria when the concentration of pyruvic acid sodium was 0.2%. Signal molecule have the smallest role.⑥Carbon metabolism board of Biolog GN was superposition and contrasted, there are six kinds of carbon can not be used after R. palustris and R. sphaeroide mixed culture, there is a deterrent effect, but these carbon can be 80 percent used when R. sphaeroide was pure cultured; R. palustris and E. aerogenes mixed culture can use three kinds of carbon, the three carbon can not be used in pure culture, metabolism pathway has changed. So from that point of ecology, anaerobic digestion bacteria on the growth of non-purple sulfur bacteria have great role in promoting and supporting. Mixed culture in the treatment of organic waste water has provided a theoretical basis as the effective use of carbon.