Study On The Development Of Molecule Interaction Analyzer Based On Surface Plasmon Resonance

The first surface plasmon resonance (SPR) sensor was developed in 1983 by Liedberg et al, since then, the research and application of SPR technology have shown extensive growth and gradually become the hot spot and research frontier in the biosensor field in the world. The most important characteristics of SPR sensor are its versatility and capability in real time monitoring the association or dissociation of biomolecules on the surface of the sensor without the need for fluorescence or labeling of the biomolecules. These characteristics made the SPR technique an easy, convenient and reliable one for determining the concentration and molecular weight, monitoring change in structure, measuring kinetic constant and binding specificity of individual biomolecules. For above reasons the SPR sensors grow as a new powerful technology in chemical and biological field.Several couplers in the SPR sensors have been employed. These include fiber optic SPR sensor, grating-based SPR sensor and prism-based SPR sensor. Based on detection mode, the SPR sensors include angle modulation, polarization state modulation, intensity modulation, phase modulation and wavelength modulation. These setup were fully discussed in Chapter one. At last some successful commercialized instruments were listed.In chapter two, a novel optical chemical sensor was developed. Traditionally, wavelength modulation SPR sensing method was to use tungsten lamp as light source which needed an expensive and complicated apparatus. The device presented here is a relatively simple and inexpensive. It was designed using white LED as light source, and lightened by a 5V battery. The setup consisted of white LED, light transmit system, sensing element, flow cell, detection system and data dealing system. The SPR sensor was designed on the basis of fixing the angle of incident light and measuring the change of resonant wavelength. The light emitted from the white LED is polarized to give TM polarized light, and two lenses are employed to make the light parallel. The parallel polychromatic light beam passes through an optical prism with a thin gold film on the bottom and excites surface plasmon at the interface between the gold film and solution. The output light from the prism is guided into a charge coupled device (CCD) detector by fiber. The data acquired by CCD was processed by data processing system.The interactions between drug and protein were presented in Chapter three. First the binding rate of antibiotics and protein was studied by this apparatus. Eight antibiotics were analyzed with the sensor. Then results were compared with the reliable binding-rate data made by standard equilibrium dialysis. The results of comparison are in good relationship and it can be used to determinate the binding rate of antibiotics and protein. Then, based on SPR, a novel optical chemical sensor for distinguishing enantiomeric drugs was developed. The difference between combination reaction of enantiomeric drugs with albumins was studied by using this setup. According to these differences the enantiomeric drugs can be distinguished. The method was shown to have many advantages over traditional methods. The major ones include the elimination of labeling and screening rapidly. Finally, also used this apparatus to carry on the heavy metal ion examination, Lyme disease's examination, has obtained good results.