Development Of The Integrated Instrument For Multi-channel Nucleic Acid Detection

Nowdays, Sanger method is commonly used for sequence detection of nucleic acid, the advantages of this method mainly lie in its strong abilities for uncharted DNA sequence analysis, long sequence detection by using unidirection reaction, etc and over 1000bp can be achieved when such a technology is utilized. However, the devices for automatic DNA sequence test based on the Sanger method are usually too expensive, and complicated. Furthermore, sequence validations are mostly carried on for known DNA sequences for which 100bp or below is enough. As a result, Sanger is not the most suitable method for short sequence analysis. Recently, the DNA sequence analysis technology based on the new-developed pyrosequencing technology is proven to be the exact method for most such applications.In this dissertation, an automatic DNA sequence detection apparatus based on the pyrosequencing technology is developed. This instrument assumes X-86 PC as Upper-Computer, AT89C51 as Down-Computer, photomultiplier (PMT) as photoelectric conversion device, HY-6022 as data collection device, Visual C++ 6.0 and keil C as programme tools. The control system is designed based on Windows platform. In the reaction process of nucleic acid with other reagents, real-time data collection, analysis and process are carried on for fluorescence signals engendered in the tubes to realize the multi-channel detection for nucleic acid sequences.Detailed software and hardware designs for the whole system are presented. Finally, we validate the function of the instrument through experiments.