Preparation And Properties Of Biomolecule Immobilized Materials

Immobilized enzyme is a type of biocatalyst, which can high-efficiently, high-selectively catalyze chemical reactions under mild conditions and doesn't pollute the environment. Enzyme immobilization is considered as one of very promising biotechnology, immobilized enzyme is of considerable interest for their applications as enzyme reactors, biological fuel cells and bio-sensors. Immobilization technology includes absorption, wrapping, covalently binding, and crosslinking methods, and so on. In this thesis, the synthesis, characterization, improment were summarized. Besides, ammo-functionalized mesostructured SiO₂ (AF-MCFs) materials and amino-functionalized mesostructured SiO₂ film were synthesized. Their application was also described. We used carbon nanoparticles to immobilize enzyme and fabricate biological fuel cells. The performance was measured. The main research work is as follows:1. Large mesopores cellular foam (LMCFs) materials with diameters ranging from 17 to 34 nm were synthesized using microemulsion templating. The amine functional groups were attached to channels of LMCFs materials via post-synthesis grafting. The structural and chemical properties of these prepared materials were characterized by TEM, XRD, FT-IR and nitrogen adsorption. The glucose oxidase (GOx) was immobilized by covalently couple enzyme molecules to the interior surface of amino-functionalized mesostructured cellular foams (AF-MCFs) materials, in which leaching of the enzyme is prevented. The immobilized enzyme exhibited the high catalytic activity and thermal stability for oxidation of glucose. It was found that GOx immobilized on AF-MCFs materials is re-useabie.2. Ordered amino-functionalized mesoporous organic-inorganic hybrid silica thin films were prepared by evaporation induced self-assembly (EISA) at acidic condition, and DNA molecules were covalently bound to these mesoporous substrates. These prepared materials were characterized by TEM. AFM. FT-IR and Laser Scanning Confocal Fluorescence Microscopy (LSCFM). The ordered mesoporous structure and highly dense covalent bonds help to increase the combining intensity and DNA density.3. We report the fabrication of a novel biofuel cell containing a glucose oxidase anode and a...