Preparation And Evaluation Of P-methylphenyl Bonded Monolithic Silica Column

Monolithic silica column is a new type column of chromatography with low column back pressure, high permeability, high mechanical strength, good performance and strong column stability which has attracted attention since 1990s. It has been widely used in catalysis, synthesis and separation fields. However, the preparation process is relatively difficult, until now only a few groups have reported it. Therefore, it is necessary to find an appropriate method about preparation and modification of the monolithic silica column.The development of the chromatographic column was reviewed at first, the preparation and modification of monolithic silica column were also introduced. Moreover, application of the monolithic silica column on HPLC was described.A p-methylphenyl-bonded silica column for high performance liquid chromatography was prepared by coupling silica with y-glycidoxypropyltrimethoxysilane to modify the stationary phase with ethanediamine to form amino-group, and then immobilizing para-toluenesulfonic acid onto the monolithic silica columns. Its' chemical characterization was confirmed by FTIR and Elemental Analysis. Its'chromatographic properties were evaluated by separating the mixture of lysozyme (Lys), human immunoglobulin (IgG) and bovine serum albumin (BSA).The results showed that the stationary phase had fine chromatographic properties and good stability. The p-methylphenyl-bonded monolithic silica column could be used for the fast-separation.Moreover, a new monolithic silica column was used to be an effective heteropoly acid H3PW12O40 (PW) support for the synthesis of bis (indoly) methanes.The present method offers short reaction times, mild condition and a recyclable catalyst with a very easy process.