D-f Metal Complex Nanoparticles And Prussian Blue Modified Fe ₃ 4 Nanoparticles In The Diagnosis And Treatment Of Integration Research

Iridium complexes have attracted more and more attention due to their excellent photophysical and photochemical properties, such as high quantum efficiency, long life time and easy to adjust the emission wavelength. As the advantages they have,phosphprescent iridium have been applied in many fields such as, biological imaging,chemical sensors, optical function nanomaterials and so on. However, the poor water solubility and biocompatibility of Ir（III） complexes in biological media limits their widely application in the living system. In this paper, we have prepared a hollow coordination polymer nanoparticle by thermal injection method, and discussed its biological imaging, magnetic resonance imaging（MRI）, ultrasound imaging and photodynamic therapy. Feridex（Fe₃O₄） has been used as negative contrast agent in medical, Prussian blue has a good effect of photothermal, in order to obtain multifunctional nanomaterials, we synthesized a Fe₃O₄ nanomaterial modified by Prussian blue. The studies include the following two parts:1. Research of d-f metal complex nanoparticles in diagnosis and treatmentWe designed and synthesized a hollow d-f metal complex nanoparticles（Ir-Dy HCPs）by thermal injection method at 110 0C in 7 hours by dialysis. Experiments show that,Ir-Dy HCPs is a new multifunctional contrast agent in Optical imaging, Photodynamic therapy, Magnetic Resonance imaging and Ultrasound imaging with good water solubility and biocompatibility. Cell experiments and confocal experiment showed our Ir-Dy HCPs have low cytotoxicity and can be swallowed into the cytoplasm;Ultrasound imaging and magnetic resonance imaging experiments in vivo and solution show that it is a very promising multifunctional contrast agent;Photodynamic therapy experiments show that it produce singlet oxygen with high efficiency. we successfully synthesized the other three hollow complex nanoparticles,such as Ir-Ho HCPs, Ir-Nd HCPs and Ir-Yb HCPs, which indicate that the method is very stable to obtain a very promising multifunctional imaging contrast agents.2.Synthesis and application of Fe₃O₄ nanoparticles modified by Prussian blue in Magnetic resonance imaging and Photothermal therapy.This study utilize the coordination between K₄[Fe（CN）₆] and Fe³⁺ on the surface of Fe₃O₄ nanoparticles to synthesize Fe₃O₄ nanoparticles modified by Prussian blue（Fe₃O₄@PB） in the presence of citric acid. MRI in solution and cell evidence that Fe₃O₄@PB still have T₂-weighted imaging function at a low concentration.Fe₃O₄@PB hace good photothermal effect in solution and cells, cell death rate was about 80% with Fe₃O₄@PB（25 μg/m L）. Fe₃O₄@PB have the cooperative properties of detection and treatment by integrate the functions of T₂-weighted imaging of Fe₃O₄ and photothermal function of Prussian blue.