Protein - Based Western Blotting Alginate Composite Hydrogel Film

Alginate acid is a kind of natural organic matter extracted from the brown seaweed. The powder of alginate acid is soluble in water, and the formed solution has a superior viscosity. Gelatification occurs when the solution containing multivalent cation is added into the solution of alginate acid. CaAlg hydrogel has the characteristics of simple preparation, low toxicity and nice biocompatibility. The fabricating process of hydrogel is mild without any organic solvent, therefore, calcium alginate hydrogel has potential application in the field of biomedicine. In addition, the mechanical properties, mass transfer process and the decomposition properties of calcium alginate hydrogel can be adjusted easily by controlling the external conditions. The object of this research is calcium alginate hydrogel and its modifying method through PP fiber and PHB fiber. The purpose of the modification was to improve the instability and echanical properties, the application of CaAlg hydrogel in protein molecular imprinting was also researched. The researches was composed of the following parts:The calcium alginate hydrogel was used as the surface molecularly imprinted materials, BSA was uses as the template. CaAlg hydrogel was characterized through SEM, FTIR. The swelling behavior and mechanical properties was researched. The influence of thickness of gel membrane type, the pH value of Tris-HCl, and the kind of the buffer on the release and adsorption capacity of BSA were discussed. The results showed that the 0.4mm gel membrane thickness showed the highest imprinting efficiency, CaAlg gel in PBS buffer solution imprinted more BSA than those in Tris-HCl buffer and NaCl solution. However, CaAlg hydrogel in PBS buffer was unstable, the excessive swelling leads to the rupture of the diaphragm. In addition, neuter Tris-HCl buffer solution is best for the imprinting process of calcium alginate Hydrogel films.PP fibers and PHB nanofibers were used as support carrier to prepare the PP-s-CaAlg gel and PHB nanofiber reinforced CaAlg hydrogel membrane, and characterized by scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR). The swelling behavior and mechanical properties was researched. The results of release and adsorption experiment results showed that the swelling ratios of PP-s-CaAlg and PHB nanofiber enhanced CaAlg were much lower than CaAlg hydrogel, but the imprinted effect was more stable, the adsorption capacity has increased, which indicated that the coating method on the fiber improved the stability and mechanical strength of CaAlg hydrogel. PP-s-CaAlg gel film and PHB nanofiber enhanced CaAlg hydrogel in PBS solution was higher than in other solution. The adsorption capacity of imprinting membrane was significantly higher than the non-imprinted membrane.