A Study On The Determination Of Illegal Additives In Food And Neurotransmitters In Biological Sample

In this paper, we explore the methods for detecting illegal additives of hydrolyzed animal protein(hydroxyproline)in dairy products and 8 monoamine neurotransmitters in biological sample.Chapter One: (1) This chapter describes the relationships of food security and disease with hydroxyproline, sums up the detection method's researches both in inland and abroad, interprets the objective and significance of the experimental study. (2) we introduce the types and function of neurotransmitters, sums up the detection method's researches both in inland and abroad,interprets the objective and significance of the experimental study.Chapter Tow: High-performance chromatography coupled to an evaporative light scatering detector was used to quantitavely determine hydrolyzed animal protein in dairy products. To investigate the effect of different mobile phase upon the separation and detection limit, the effect of drift temperature and carrier gas pressure upon the final target detection result. The results show that We have exploited the hydroxyproline content of hydrolyzed animal protein and developed a method for hydroxyproline quantification as a measure of hydrolyzed animal protein content in dairy products. There is no need to derive in the analytical method ,that can avoid derivatixe diversity and the problem of derivatixe not thoroughly of amino acid in sample .The method is simple, rapid (5 min), precise, accurate, sensitive and can be applied to the determination of hydrolyzed animal protein with satisfactory results.Chapter Three: High-performance chromatography coupled to an fluorescence detector(HPLC-ELSD)was used to simultaneously determine 8 monoamine neurotransmitters and metabolites in different sections of mouse brains. To investigate the effect of the different mobile phase composition,the parameter of detector,the condition of sample's pretreatment on the final target detection result. The results show that 8 monoamine neurotransmitters and metabolites can be separated and detected under the same mobile phase, mono-flow rate and isocratic elution.The Kinematica homogenizer take the place of the manual operation's homogenizer was used to prepared homogenate of brain tissue,and found out the firavvfirst available pretreatment of sample.The advantages of the method include easy and prompt operation,high recovery,low detection limit,good separation effect,high accuracy and precision. Chapter Four: High-performance chromatography coupled to an fluorescence detector(HPLC-RF)was used to simultaneously determine 8 monoamine neurotransmitters and metabolites in human serum. Quantitavely determine 8 monoamine neurotransmitters in human serum on the base of the chapter three. The results show that the advantages of the method includes easy and prompt operation, high recovery, low detection limit, good separation effect, high accuracy and precision. The method has practical value for detecting 8 kinds of monoamine neural transmitter and its metabolites in biological samples,that can provide base to diseases diagnosis,drug therapy and basic research.