The Study On Analysis And Degradation Of Typical Steroid Estrogens In Animal Waste During Land Use

The pollution of environmental estrogens has become another global environmental proble-m, which follows the ozone layer destruction and greenhouse effect.More and more attention all over the world has been paid on the research and treatment of environmental estrogens. Theref-ore, it is very important to conduct some related studies, including identification and monitoring ecological effect and pollution control of environmental estrogens.First, Greater potential harm in pig manure was found in estrone (El),17a-estradiol (aE2), 17β-estradiol (βE2) and estriol (E3), a gas chromatography/mass spectrometry method was dev-elopped for the confirmed identification and quantitation of the four estrogens in pig manure sa-ples.Foucus on the optimization of pre-treatment method including analyzing the influence of the ultrasonic extraction eluent, extraction time, solid phase extraction eluent, deri-vatization temp-eratures and times on the four typical steroid estrogens testing in pig manure. The results showed that the detection limits for E,αE2,βE2 and E3 in pig manure were 8.0,2.5,5.1 and 3.3μg/kg under the optimal experimental conditions, the average recoveries were in the range of 92.3%～137.4%, and also showed that this method in practical testing is feasible.Secondly, batch experiments investigated the effects of sodium azide,light treatment,temperature and composite of tween 80 or tylosin using soils amended by cow,pig or chicken manures spiked with E1 and E2 during natural degradation. The results showed that sodium azide could significantly inhibit the degradation of E1 while had little effect on the degradation of E2, furthermore, it also proved that biodegradation is the primary degradation of E1; It indicated that the contribution of light treatment of E1 and E2 is limited; and both El and E2 under natural degradation are unacted on the influence of manure types; Range between 0 and 35℃,the degradation rates of E1 and E2 increased with the rise of temperature, degradation rate of E1 in soil improved of cow manure rose from 1.21%to 58.04%while the degradation rates of E2 increased from 1.24%to 48.93%,it proved that high temperature promote degradation speed of E1 and E2 obviously; Environment coexistence of Tween 80 can promote the degradation of E1 and E2;When coexistence with Tylosin and Tween 80, it can effectively promote the degradation of E2.Thirdly, this paper explores the compost process of E2 using cow,pig and chicken manures under aerobic,anaerobic or aerobic and anaerobic alternately conditions.It was found that degradation of E2 in different conditions accord with primary dynamic rules; fastest degradation rate was more than 90%,it was found in cow manure under aerobic compost; E2 degradation in the composting process produces a by-product of E1; the concentration of E1 after 60 days can not be detected; During aerobic composting, the concertration of E2 increased at first and then decreased,which gave a peak-shape curve; when under anaerobic/aerobic and anaerobic condition, concentration of E2 first increased then decreased, then increased until can not detected, it signified that aerobic compost using animal manures is a efficacious way to remove E2, and there was not any toxic byproduct, meanwhile animal manures after composting can reach the standard of agricultural green manures.Finally,this study may provide theoretical support to better understanding the estrogens in environmental behavior, and also provide a theoretical and technical support for planning poli-cies and regulations of livestock manures pollution risk management and future development, in a word, it has an important practical significance.