Process And Mechanism Of Inclusion With Cinnamaldehyde And Its Inclusive Performance

Encapsulation technology has been remarkable developed in the last years for the design and formulation procedure for the stabilization of activie components. A relevant method that has been investigated for the encapsulation to improve high value-added products is the utilization of beta-cyclodextrin and chitosan.Cinnamaldehyde is an essential oil constituent occurring strong antimicrobial activity, which is widely applied in pharmaceutical, cosmetic and food industry. The Encapsulation technology can significantly enhance stability and retention of cinnamaldehyde.β-cyclodextrin (β-CD) is a suitable microencapsulation coating for cinnamaldehyde owning to its unique structure. It has a hollow truncated cone shape that is hydrophilic at the periphery and hydrophobic in the central cavity.β-CD can form inclusion complexes with different guest molecules in aqueous solution or in the solid state, especially with benzene-containing guest molecular.The aim of this research is to obtainβ-CD-cinnamaldehyde inclusion complexes with high yield, oil content, and complex content, by optimizing the process of grinding method, saturated aqueous method and spray drying method. The results show that ethanol can accelerate singnificantly mass transfer of inclusion as a co-solvent, and increase the yield and complex content of inclusion complex. The high-temperature release experiment confirm thatβ-CD has a very strong controlled release effect on cinnamaldehyde. TG-DSC, XRD, FT-IR and 13C-CP/MAS NMR and in-situ infrared experiment were utilized to analyze the structure and inclusion process ofβ-CD-cinnamaldehyde inclusion complex. PM3 and the two-layered hybrid ONIOM methods were applied to optimize the geometry and simulate the inclusion process. A clear description for the stable structure of the cinnamadehyde-β-CD inclusion complex was obtained:the benzene ring of cinnamaldehyde inserted into the cavity ofβ-CD while the aldehyde group interacted with the secondary hydroxyls which was located in the wide side of theβ-CD. Furthermore, the results of the solvation effect (Onsager) indicated that ethanol led to more stable inclusion complexation. This coincided with higher yield and complex content, which could be obtained by addition of ethanol during the process. Experimental and theoretical approach to investigateβ-CD inclusion complex is feasible, such investigation maybe significant for the applications of preparing new inclusion complex.