The Impact Of Submerged Vegetation Restoration On The Structure Of Bacterioplankton In Huizhou West Lake

Macrophytes play a key role in in lake ecosystems, especially in shallow lake ecosystems. They can not only absorb nutrients competing with phytoplankton, and inhibit the growth of phytoplankton by secreting allelochemicals, but also can change the physical and chemical conditions of lake ecosystems. Bacterioplankton are also very important parts of the lake ecosystems, who have great importance in biogeochemical cycles. It becomes easier to study the impact of submerged vegetation restoration on the structure of bacterioplankton for the fast developing of molecular biology techniques.Restoration of submerged vegetation in shallow eutrophic lake is an important method of lake restoration, some areas of Huizhou West Lake has been restored successfully by planting submerged plants, and the water has been improved significantly. In order to study the impact of the submerged vegetation on bacterioplankton, bacterioplankton community composition was investigated in restored area and un-restored area in Huizhou West Lake. The composition and diversity of bacterioplankton of the restored area and un-restored area was analyzed by denaturing gradient gel electrophoresis (DGGE) and constructing 16S rRNA gene clone library, using the bacterial 16sRNA gene universal primer to do the polymerase chain reaction. The results showed that Pielou index and Shannon-Wiener index of the restored area were significantly higher than that of the un-restored area. The compositions of bacterioplankton community were significantly different between restored and un-restored area, the Proteobacteria ratio and diversity of restored area were higher than that of un-restored area. The study indicated that submerged vegegtaion can not only change and improve water quality, but also can increase the diversity of bacterioplankton.