The Controllable Synthesis Of Typical Inorganic Metallic Nanoparticles And Their Applications In The Analysis Of Biochemical Pharmaceuticals

As inorganic metallic nanomaterials are of unique properties in optics, electricity, magnetics, and catalysis, they have been widely applied in the fields of environment monitoring, food safe analysis, cell or tissues imaging, and catalysis. As a result of this, based on the difference of optical property of two typical inorganic metallic nanomaterials with disparate diameter and configuration:CdTe quantum dots and gold nanoparticles capped by trisodium citrate which have been utilized abroad in theoretical research, we design two methods based on "turn-on" fluorescence mode and accomplish the detection of thiol-containing pharmaceutical (take the 6-Mercaptopurine as an example). Meanwhile, we utilise central nervine transmitter dopamine as a reducing and coating reagent to synthesize a kind of gold material with complicated three-dimensional configuration, and then do the research of its optical property and its special reaction with halogenic ion, the main three points are as follows:1,Sensitive turn-on fluorescent detection of 6-Mercaptopurine base on fluorescence of quantum dots. Quantum dots has excellent fluorescent property, because cupric ion can induce quantum dots aggregate with carboxy on the surface, and quench its fluorescence, but in the present of compound with thiol group, it can reduce cupric to cuprous and form disulfur bond on itself, thus the fluorescence of quantum dots resumes gradually. On the basis of this, we present a non-label, high-speed and sensitive detection of thiol-containing pharmaceutical (take 6-Mercaptopurine as an example).2,Sensitive turn-on fluorescent detection of 6-Mercaptopurine based on surface energy transfer between gold nanoparticles and Rhodamine B. As gold nanoparticles have immense extinction coefficient, cationic dye Rhodamine B can attract to the surface of gold nanoparticles with negative charge through electrostatic interaction, when excited the fluorescent energy of the stimulated Rhodamine B thansfers to gold nanoparticles, result in fluorescence quenching. With the addition of some compound which contains thiol group, because of stronger gold-sulfur (Au-S) covalent bond, drives the cationic dye Rhodamine B get rid of gold nanoparticles'surface and release into the solution, reducing the energy thansfer efficiency, and the fluorescence of the system resume gradually. On the basis of this, we establish a high-speed and sensitive detection of thiol-containing pharmaceutical (take 6-Mercaptopurine as an example).3,Envionment-friendly rount to synthesize three-dimensional flower-shaped gold microparticles. Using solution-phase synthesis route, we applied central nervine transmitter dopamine as a reducing and coating reagent, and we found a green and facile method to synthesize a kind of gold material with flower-shaped, then we study its property in optics and research the reciprocity reaction between the flower-shaped gold microparticles and familiar anion, especially halogenic ion.