Metal Matrix Nano-materials For The NO Donor Drug Development

Nitric oxide is a novel biological messenger molecule, involved in many physiological and pathological activities. Recently, a large number of studies have shown that many pathological process of the human body is closely related to NO levels. May be too low or too high levels of NO produce a lot of pathological effects. Therefore, NO regulator research has become one of the hot new drugs.S-nitrosoglutathione (GSNO) as NO donor and transporter, often used as a Prodrug of NO. When the NO releasing from GSNO, the catalyst required to participate. Therefore, the development of a catalyst can be controlled catalytic GSNO release NO is very important to sustained-release drugs of NO. In this thesis, because of the unique size effect of nano particles have shown good catalytic activity, carried out based on nano-particle drug release of NO research. Specific measures include the following aspects:1. Gold Nanoparticles catalytic GSNO release NOSodium citrate reduction of the classical gold nanoparticles were prepared,using transmission electron microscopy and ultraviolet spectroscopy to characterize its performance. Using electrochemical method detect gold nanoparticles catalyzed the release of NO in GSNO, the results showed that gold nanoparticles have a good catalytic performance, and the role of gold nanoparticles on the release of NO in GSNO in a concentration dependent, further characterization of its reaction products shown that the occurrence of this reaction is due to Au-S bond formation.2. Chitosan gold nanoparticles wrapped controlled release of NO regulation of GSNOWater-soluble O-hydroxyethyl chitosan nanoparticles was prepared, with different concentrations of chitosan nanoparticles and gold nanoparticles interact and then prepared gold nanoparticles coated by chitosan. Electrochemical methods and ESR methods were used to study this particles on the release of NO in the regulation of GSNO. The results showed that gold nanoparticles wrapped with chitosan nanoparticles make the release of NO in GSNO slow, and its catalytic efficiency decrease with the concentration of chitosan increases.3. Different ligand-protected gold nanoparticles on the release of NO of GSNOOn the basis of gold nanoparticles protected by sodium citrate, prepared gold nanoparticles protected by ligand exchange of amino and thiol-protected. Electrochemical method compared three different ligand-protected gold nanoparticles the efficiency of catalvtic GNSO release of NO. The results showed that when the force reduction between ligand and gold nanoparticles or surface coverage ratio of ligand increases, the catalytic efficiency of gold nanoparticles decreases.4. Different types of nanoparticles catalytic GSNO release NOSodium citrate as a protective agent, sodium borohydride as a reducing agent, were prepared by gold, silver, platinum nanoparticles. TEM and UV-Vis spectroscopy to characterize its performance, and then compared there catalytic efficiency of GSNO release NO by using electrochemical mathod. The results showed that silver nanoparticles and gold nanoparticles have a high efficiency and there catalytic properties are similar to each other. The catalytic efficiency of platinum nanoparticles is very low.