Base By Reducing Microbial Agents In Hong Improve The Quality Of Research And Application Of Tobacco

Nicotine is a harmful substance, harmful to human health, how to reduce the nicotine content of tobacco has been the focus of world, physical and chemical methods can be reduced very low nicotine content, but also reduce the aroma of the content and quality.Thesis aroma from the lower base isolation and identification of microorganisms, selection, culture conditions of high strain, antimicrobial action mechanism and use of research, field application, after the agents dealing with tobacco redrying Natural Aging sensory evaluation, antimicrobial treatment enter "Yuxi" brand cigarettes smoke after the impact of the quality, reduce Nicotine Tobacco Hong Fungi of aroma components in terms of these micro-organisms from the lower base aroma to the application of mechanisms.Main results:1, lower base isolation and identification of microorganisms by Hong:the project through the enrichment culture, the use of nicotine as the sole carbon source in selective medium, from the tobacco, tobacco factory waste water samples and soil samples down to get more than 2680 base line strain.The 16S rRNA sequence analysis and determination of physiological and biochemical properties, isolated from the leaf nornicotine on bacteria mainly Pseudomonas, base down in the soil bacteria were the dominant species Arthrobacter.2, the lower base aroma microbial screening:In the early and burley tobacco curing before spraying on the leaf surface, Arthrobacter, prepared after the determination of chemical components and internal quality changes.The strains of burley tobacco nicotine content of different parts of the most significant, can nicotine degradation rate of 40% or more, up to 54.22%, also up to 7.12% minimum.3, lower base fine aroma microbial culture conditions of strain:the 4 screened,9 strains of the culture conditions were optimized to fit 4 and 9 of the culture conditions were 30℃, pH 6.5,48 h and 35℃, pH 6.5,56 h.Optimization of fermentation medium and fermentation conditions for screening applications for these two strains of laying a foundation.4, lower base aroma mechanism of action of microbial agents and the use of methods:The strains of burley tobacco nicotine content of different parts of the most significant, can nicotine degradation rate of 40% or more, up to 54.22%, the lowest also up to 7.12%.Through different application methods of the experiment, we believe that before the application of reduced tobacco baked aroma of alkali viable bacteria is a reliable and simple preparation to use.5, lower base aroma strain field application:bacterial treatment, the tobacco aroma with different degrees of increase in the amount of middle leaves the greatest impact aroma, but no significant difference between the two strains.In terms of aroma and taste, the use of strains, the smoke fine, reduce irritation, decreased strength and finish improvements.6, after the agents handling redrying Natural Aging Sensory Evaluation of Tobacco:After 4 and 9, pre-baked tobacco and 1 year after natural redrying Aging was conducted after the evaluation of sensory quality, the quality is superior to all parts of tobacco control tobacco.7, the agents handle into the "Yuxi" brand cigarette smoke after the impact of quality:Smoking identified by experts, dealing with the cigarette agents to handle samples thick aroma Fu elegant, rich and full aroma Zhixi Ni, harmonization is good, No odor, no irritation, clean finish, comfort, smoking score higher than the control.8, reducing Bacteria for Treatment of Nicotine Tobacco Hong analysis of aroma components:aroma components analysis showed that, after antimicrobial treatment of tobacco content of aroma components changed to improve the amount of tobacco aroma and aroma quality, reducing the nicotine content, and improved leaf quality, improve the availability of tobacco.