The Detection And Significance Of Calcified Nano-particles In The Surface Encrustation Deposition Of Double J Ureteral Stent

Objective::To compare the distribution of Calcified nano-particles in the double "J" catheter that remained some time in the human body in different ways,and investing at the role of Calcified nano-particles formed in the catheter surface.Methods:Collect 36 double "J" catheters remained 4 weeks after nephro-ureteral calculus operation from Urology Cystoscopy Room. Use transmission electron microscopy and PCR to detect distribution of Calcified nano-particles in the double "J" catheters, and then compare the differences between the two methods. All of samples were cut out both ends on the sterile console, one was detected by electron microscopy, and the other was handled as following: After de-mineralization, it should be cultured in fetal calf serum DMEM, then after 4-8 weeks, they were identified by separating, scanning electron microscopy and extracting genomic DNA, Calcified nano-particles were amplificated 16SrRNA gene by PCR . Data was analyzed by SPSS 16.0 statistical analysis software package, and P<0.05 was regarded to be statistically significant.Results::With electron microscopy , we observed nano-particles in the surface of 20 cases double "J" tubes, and the other 16 cases were not found. While with PCR, there were 28 calcified encrustation nano-tubes expressing 16SrRNA positive, only 8 cases were negtive. The comparison of the two groups showedχ2 = 4.000, P = 0.046, with statistical significance.Conclusions:PCR has a certain sense in detecting calcifying nano-particles in the surface of double "J" tubes , and it is superior to electron microscopy. The calcified nano-particles exist in the scaling surface of double "J" catheter, and it may play an important role in the formation of surface encrustation.