Study On The Preparation Technology Of γ-Aminobutyric Acid And Ceftriaxone Sodium Liposomes

γ-aminobutyric acid is not only a non-protein amino acid which widely distributed in plants and animals but also is an inhibitory neurotransmitter that present in mammalian brain and spinal cord. In the human or animal body, it can be used as inhibitor to prevent the rise of blood pressure and also can protect brain and improve brain function and so on involved in physical activities in cerebral circulation. Ceftriaxone Sodium, a third generation cephalosporin, which is very sensitive to the vast majority of Gram-positive bacteria and negative bacteria, personification so many characteristics, such as low toxicity and resistance to enzymes, strong penetration in tissue and long elimination of half-life, is one of cephalosporin that has the longest half-life currently. It has achieved significant effect in clinically for the treatment of infection in the lower respiratory tract, and skin as well as in bacterial septicemia.In this paper, we mainly studied on theγ-aminobutyric acid liposome and ceftriaxone liposome. On the basis of the methods for preparing liposome and measuring encapsulation, one-factor tests and four factors, three-level orthogonal experimental design were carried out to choose the level of various factors, using the range analysis to obtain the optimal preparation conditions of liposome ultimately .The calcium acetate gradient method applied to prepareγ-aminobutyric acid liposome, through the combination of one-factor tests and orthogonal tests, the optimum conditions were determined as followed:γ-aminobutyric acid liposome /egg phosphatidyl choline (EPC)1:20, egg phosphatidyl choline(EPC)/clearance(CL)4:1, concentration of Calcium acetate was 0.12 mol/L, incubation temperature 50℃.Under the optimal formulation,γ-aminobutyric acid liposome was encapsulated 91.4%. Through the reverse-phase evaporation method, one optimum recipes of Ceftriaxone liposome was found that it showed Ceftriaxone liposome/EPC 1:10, Ceftriaxone liposome/EPC 1:4, incubation temperature 55℃, hydration medium (Tris-HCl buffer) was added by the volume of 20 mL, Under the optimal formulation, Ceftriaxone liposome was encapsulated 31.26%.The liposome shows partical in the shape of pellet or elliptical shape and distribution of size uniformity under transmission electron microscopy. The average diameter ofγ-aminobutyric acid liposome and Ceftriaxone liposome are about 55 nm and 45 nm respectively.At 4℃and 25℃, the physical and chemical stabilities ofγ-aminobutyric acid and Ceftriaxone liposomes were investigated by observing liposome morphology and measuring the changes of diameter, leakage rate and the malondialdehyde(MDA) content. At 25℃, with the time going up, the particals would assemble, the leakage rate would increase and the lecithin would be oxidated, MDA would increase in the value. However, at 4℃, the stability of liposome can be improved such as in the inhibition the aggregation of liposome and slowing down the leakage of material contained in the liposome as well as the increaseing in MDA values.