| Objective:Establishing the quality method of total flavonoids and quercetin content, optimizing preparation technology, studing on the quality standard and HPLC fingerprint of guava leaf.Methods:①The total flavoniods in guava leaf were determined by UV-spectrophotometry with rutin standard and NaNO2-AlCl3-NaOH system, the content of quercetin was determind by HPLC.②By single-factor and orthogonal experment methods, the optimal extration technology and formation technology were determined. Based on the above results, quality control of semi-finished produce and small experiment were discussed.③In the studies on its quality standard,the TLC, HPLC methods were established to identify the guava leaf granule. Meanwhile, the similarity evaluation system for chromatographic fingerprint of TCM(2004 A edition) was used to evaluate the similarity of the 10 batches of guava leaf granule.Restults:①The good linear range waried from 00.048mg/ml(r=0.999), the average recovery was 99.49% for total flavonoids, assayed by UV with the standard sample of rutin. And the good linear range varied from 0.1161.856μg(r=0.9999), the average recovery was 99.38% for quercetin, assayed by HPLC.②The optimum preparation process was as follows: extracted it with 8 times 70% ethanol for 3 times, 1hour in turn. Concentrated the mixed decoction to 1.2(60℃). And precipitated by 10 times water laying for 12 times, filtrated and concentrated it to 1.1(60℃),dry it by spray drying(the inlet temperature was 180℃, the feed flow rate was 70%), after that, we can select the granulation condition(dry extract:soluble starch:aspartame=1:1:0.2)③Three expansion methods indicated that, we can get the corresponding spot of the testing and quercetin standard at the corrrsponding locations in the TLC, the methodological study showed that this method is highly credible. Conclusion: All of these studies indicade that the preparation procedure is reasonable and practical, the standard is controllable, the quality of the sample is steady on the whole as well. |
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