Study On Extraction Of RNA From Glutamate Wastewater

China is the largest producer of monosodium glutamate, MSG are produced more than 700,000 tons annually. It is the first in the world, The pollution of monosodium glutamate is high and will cause serious result. According to statistics, each 1 ton of MSG is produced, 20 tons of waste can be discharged to the environment. Glutamate wastewater contains large amounts of proteins as well as useful organic, full using of these proteins will be turned waste into wealth. Therefore, the study on extraction of RNA from cell protein is extremely important to solve the current RNA shortage of raw materials in China. This issue can not only remove part of the organic waste, but also reduce the pollution of waste to the environment, while producing high quality products. It will be certain economic and social benefits.In this paper, I explore glutamate wastewater as raw materials, and carry out the following aspects of work:First of all, glutamic acid was determined using a variety of wastewater composition and content. Total acid, density, residual glutamate, CODcr, protein, amino nitrogen, reducing sugar, total solid, free fat, ash, content of wastewater is 0.9984 mL/100mL, 1.057×103 mg/L, 290 mg/dL, 20496 mg/L, 7.67 mg/mL, 0.25%, 0.973g/100mL, 0.82g/100mL, 0.0972 g/mL, 2.3%, 0.5%.Secondly: the flocculant is sodium polyacrylate used to extract cell protein. By studying the single factor and orthogonal experiments of addition of sodium polyacrylate, temperature, time, stirring speed, receiving the optimum extraction conditions were: pH of reaction system was 3.0 close to the original wastewater, the reaction temperature was 60℃, sodium polyacrylate was 2%, stirring speed was 20 r/min, mixing time was 15 min, extraction rate of cell protein was up to 85%, the time of sedimentation settling was 5 min.Thirdly: the cell protein extracted out of the waste water was as raw materials, salt, and alkaline autolysis ultrasonic method was used, by single factor experiments and orthogonal test to determine the RNA extraction. Experiments show that: The optimal conditions salt extraction RNA were: The pH, time, temperature of extraction were 9.0, 105 min, 95℃, concentration of NaCl was 6%. The optimal conditions were ultrasonic extraction of RNA: The power of ultrasound was 200W, the time of ultrasound was 40 min, ratio of solid to liquid was 1:10. Lastly: the RNA extracted was purified, One method of the proteins removaling was used by enzymatic, The optimum conditions of enzymatic were: dosage of enzyme 0.5%, reaction time 1h, hydrolysis temperature 55℃, pH 6.0. At this condition, the removal rate of protein was 60.2% and the loss of RNA was 10%. The purity of RNA determination by UV absorption spectrometry was 89.47%.