Recognition Characterization And Application Of Co (II) Fluoroquinolones Imprinted Polymer

A novel molecularly imprinted adsorbent with highly selective recognition capability in aqueous phase had been investigated for selective separation and enrichment of toxic and hazardous substances, which is of importance to develop analytical methodology, control food safety and ensure human health.In this work, the metal complex imprinted polymer of lomefloxacin, enrofloxacin and sarafloxacin was synthesized in the polar solvent and aqueous phase using metal coordination interaction. The selective recognition characterization and mechanism the molecular imprinted polymers of lomefloxacin, enrofloxacin and sarafloxacin in aqueous phase was detailly studied. The effect of various factors on the adsorption capacity was investigated. A new concept of the synergetic recognition on template molecule by organic acids with the imprinted polymer was proposed. The metal complex imprinted polymer was used as solid-phase extraction (SPE) sorbent for preconcentration and determination of the fluoroquinolones residues from biology sample by SPE-HPLC.Chapter 1: The introduction of the dangers of fluoroquinolone residues and its detection methods. The application of solid-phase extraction to separate and detect the fluoroquinolones residues, and the progress of metal complex molecularly imprinted polymers were reviewed.Chapter 2: The metal complex imprinted polymer of lomefloxacin was synthesized in the aqueous phase using metal coordination interaction. The interaction between the functional monomer and template, coordination mechanism of the metal ion and the recognition mechanism was studied by the ultraviolet and infrared. Under the optimal solid phase extract condition, the selective, dynamic adsorption and purification was studied by HPLC-UV. The recoveries of three FQs of OFL, LOM and CIP for the spiked urine samples were 84.0-96.5%,94.3-103.5%,81.9-96.5%.Chapter 3: The molecularly imprinted polymers (MIP) of Co(II)-enrofloxacin were synthesized in methanol. The results of equilibrium binding experiments show that the recognition performance of the MIP were effected by the concentration of metal ions, pH, the different anion and cation. The results of Scatchard analysis show that two classes of binding sites existed in the metal complex imprinted polymers, with their maximum apparent binding capacity estimated to be 102.76μmol g-1 and 65.23μmol g-1, respectively. It was applied to solid-phase extraction-HPLC of OFL, LOM and ENR in fish sample. The recoveries of OFL, LOM and ENR were 86.3%, 86.5%, 92.3%.Chapter 4: Using cobalt(II)-sarafloxacin complex as template, a new kind of metal-complexing template polymer, which has the binding sites analous to metal-chelate antibody, was prepared in aqueous media. The mediate role of metal ions for the selective recognition of LOM in aqueous phase and the influences of the organic acids on the recognition performance of the MIP were investigated. The study found that organic acids can obviously increase the binding capacity of MIP and a new concept of the synergetic recognition on template molecule by organic acids with the imprinted polymer was proposed.