Determination Of Glutathione In Lycium Barbarum L.

In this paper, the following four chapters will be discussed in detail: Chapter 1 IntroductionThe nature and biological function of glutathione were reviewed. Point out the importance of glutathione research, Introduction this paper's topics ideas and research content. Chapter 2 UV-Vis Spectrometric and Mass Spectrometric Determination of GSH in Wolfberry Using DTNB as Derivatizing ReagentReduced glutathione (GSH) in fruit of Lycium Barbarum L. has been determined by mass pectrometry and UV-Vis spectrometry using 5,5'-dithio-bis-nitrobenzoic acid (DTNB) as a semultaneous derivatizing agent. The results indicted that GSH in fruit of Lycium Barbarum L. significantly influenced by the chemical environment out of the cell during sample tratment, which leads to the difficulty to determine the GSH content in the sample cells. Extraction solvents with DTNB as a simultaneous derivatizing agent inhibites transformation or decompose of GSH to other products because of immediate derivatization during sample treatment. ESI-Mass spectrometry and UV-Vis spectrometry was employed to determine GSH qualitatively and quantitatively in the sample. The results show that fruit of Lycium Barbarum L. containes GSH in the range of 6.56-7.37 mg/g, which is one of the most GSH abundant plant species so far reported in the literatures. Chapter 3 Fluorescence Spectrometric Determination of GSH in Lycium Barbarum L. Using O-phthaldehyde as Derivatizing ReagentUsing ESI-Mass spectrometry and UV-Vis spectrometry of the o-phthalaldehyde (OPA) and glutathione (GSH) fluorescence derivatization, OPA found that GSH and pH= 8 is generated in two different structure derivative product. One with the GSH thiol and amino and aldehyde condensation products directly send a strong fluorescence emission and its fluorescence emission intensity and the concentration of GSH was a good linear relationship. Fluorescence spectrometry using standard curve method and standard addition method Lycium Barbarum L. extract the GSH content was found to date in Lycium Barbarum L. plant samples containing the highest content of GSH determination. In this study, based on previous work Determination of GSH fluorescence methodology and derivation of the reaction mechanism are made further in-depth discussion and for the first time determination of GSH content in Lycium Barbarum L. Medicinal plants was found by measuring the Lycium Barbarum L. is a plant rich in GSH. The study of biological activity and GSH in the Lycium Barbarum L. in the evaluation of medicinal value and the Lycium Barbarum L. In both disease prevention and control of the application of practical significance. Chapter 4 Electrochemical Determination of Glutathione (GSH) Using complexation between Glutathione(GSH) and copper ion.This paper discribes a indirect determination method of glutathione using cyclic voltammetric determination according to the complexation interaction between copper ion and GSH. In the extra copper ion solutions, anodic stripping currents of copper is linearly decreased as the GSH concentration increased. The method is reproducible and sensitive with RSD 0.5132%(7 paralel determination) and detection limit 5.6×10-7 mol·L-1. Satisfactory results were botained by the determination of GSH in the injection sample and the results is correspondant with the result determined by UV-Vis spectrometric methods.