Study Of Naphthalene On The Effect Of Developmental Toxicity Of Zebrafish (Danio Rerio) Embryo

As the long-term fuel combustion, oil spills and other anthropogenic emissions, PAHs (Polycyclic Aromatic Hydrocarbons, referred as PAHs) have posed a serious threat to the natural environment and human health. Naphthalene(NAP), persistent organic pollutants, distribute widely, which has phototoxic effects. The research on its toxicity has become an issue of global concern.In this paper, zebrafish(Danio rerio) embryos and larvae were selected as the test bodies. We used the technical of embryonic development to study the toxicity of naphthalene on zebrafish embryonic development. On the base of acute toxicity test on naphthalene, we've assessed the aquatic toxicity of naphthalene by sub-lethal toxicity and teratogenicity. The result showed that the median lethal naphthalene concentration (LC50) in embryonic acute toxicity tests in 24h,48h were 10.04 mg/L,8.96 mg/L.Naphthalene led to the problem of lethal and sublethal effects in zebrafish embryos development, including fetal autolysis, stagnant growth, developmental abnormalities, non-autonomous activity within 20s, the significant reduction of heart rate, melanoma cell aplasia, delayed hatching,72h,malformations.The most sensitive indicator was deformity within 72h, the EC50 value was 10.64 mg/L. Symptoms included yolk edema, pericardial edema (linear distortion of heart), and the spinal flection.On the base of the embryo toxicity test, we've used the biopsy technique to study naphthalene toxicity on the level of tissue and organ. When zebrafish larvae were exposed on naphthalene for 3d, the symptoms were mainly in the yolk sac, pericardium, and spinal deformity on the three organs.